| A Polysaccharide Isolated from Ecklonia cava Fermented by Lactobacillus brevis Inhibits the Inflammatory Response by Suppressing the Activation of Nuclear Factor-κB in Lipopolysaccharide-Induced RAW 264.7 Macrophages. | |
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MedLine Citation:
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PMID: 22145773 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract We previously reported that the increment of carbohydrate content in the Viscozyme(®) L (Novozyme Corp., Oklahoma City, OK, USA) extract of Lactobacillus brevis-fermented Ecklonia cava affected the inhibition of nitric oxide (NO) production and that it might be related to the polysaccharide compound. However, there is no report of anti-inflammatory effects of the polysaccharide or its biological mechanism. Here, we investigated the anti-inflammatory effects of the polysaccharide and its biological mechanism in lipopolysaccharide (LPS)-activated RAW 264.7 cells. The polysaccharide isolated from the Viscozyme extract of L. brevis-fermented E. cava (VLFEP) dose-dependently decreased LPS-stimulated NO production without cytotoxicity. Also, VLFEP significantly decreased the production of prostaglandin E(2) (PGE(2)) at the 100 μg/mL concentration. In addition, VLFEP dose-dependently decreased the protein and mRNA expressions of inducible NO synthase, whereas it slightly decreased those of cyclooxygenase 2 and only at the 100 μg/mL concentration. Moreover, VLEFP dose-dependently decreased the productions and/or mRNA expressions of tumor necrosis factor-α and interleukin-6, compared with those of LPS only-stimulated cells. In further experiments, VLFEP considerably reduced the phosphorylation and degradation of inhibitory κB as well as the translocation of nuclear transcription factor-κB (NF-κB) p65 into the nucleus, and its DNA binding was markedly induced by LPS stimulation. This study suggests that VLFEP exerts anti-inflammatory effects by down-regulating the production and expression of pro-inflammatory cytokines and mediators via inhibiting the NF-κB pathway in LPS-stimulated RAW 264.7 cells. |
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Authors:
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Won-Woo Lee; Ginnae Ahn; Janaka Priyalal Wijesinghe Arachchillage; Young Mog Kim; Se-Kwon Kim; Bae-Jin Lee; You-Jin Jeon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medicinal food Volume: 14 ISSN: 1557-7600 ISO Abbreviation: J Med Food Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812512 Medline TA: J Med Food Country: United States |
Other Details:
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Languages: eng Pagination: 1546-53 Citation Subset: IM |
Affiliation:
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1 Department of Marine Life Science, Jeju National University , Jeju, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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