Document Detail

Polypyrrole solid phase microextraction: A new approach to rapid sample preparation for the monitoring of antibiotic drugs.
MedLine Citation:
PMID:  20441869     Owner:  NLM     Status:  MEDLINE    
Simple or even rapid bioanalytical methods are rare, since they generally involve complicated, time-consuming sample preparation from the biological matrices like LLE or SPE. SPME provides a promising approach to overcome these limitations. The full potential of this innovative technique for medical diagnostics, pharmacotherapy or biochemistry has not been tapped yet. In-house manufactured SPME probes with polypyrrole (PPy) coating were evaluated using three antibiotics of high clinical relevance - linezolid, daptomycin, and moxifloxacin - from PBS, plasma, and whole blood. The PPy coating was characterised by scanning electron microscopy. Influences of pH, inorganic salt, and blood anticoagulants were studied for optimum performance. Extraction yields were determined from stagnant media as well as re-circulating human blood using the heart-and-lung machine model system. The PPy-SPME fibres showed high extraction yields, particularly regarding linezolid. The reproducibility of the method was optimised to achieve RSDs of 9% or 17% and 7% for SPME from stagnant or re-circulating blood using fresh and re-used fibres, respectively. The PPy-SPME approach was demonstrated to meet the requirements of therapeutic monitoring of the drugs tested, even from re-circulating blood at physiological flow rates. SPME represents a rapid and simple dual-step procedure with potency to significantly reduce the effort and expenditure of complicated sample preparations in biomedical analysis.
Malgorzata Szultka; Ricarda Kegler; Patricia Fuchs; Pawel Olszowy; Wolfram Miekisch; Jochen K Schubert; Boguslaw Buszewski; Ralf G Mundkowski
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Publication Detail:
Type:  Journal Article     Date:  2010-04-10
Journal Detail:
Title:  Analytica chimica acta     Volume:  667     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-08-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus, Copernicus University, Gagarin 7, 87-100 Torun, Poland.
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MeSH Terms
Acetamides / analysis,  blood,  isolation & purification
Anti-Bacterial Agents / analysis*,  blood,  isolation & purification
Anticoagulants / chemistry
Aza Compounds / analysis,  blood,  isolation & purification
Chromatography, High Pressure Liquid
Daptomycin / analysis,  blood,  isolation & purification
Hydrogen-Ion Concentration
Oxazolidinones / analysis,  blood,  isolation & purification
Polymers / chemistry*
Pyrroles / chemistry*
Quinolines / analysis,  blood,  isolation & purification
Salts / chemistry
Solid Phase Microextraction / methods*
Reg. No./Substance:
0/Acetamides; 0/Anti-Bacterial Agents; 0/Anticoagulants; 0/Aza Compounds; 0/Oxazolidinones; 0/Polymers; 0/Pyrroles; 0/Quinolines; 0/Salts; 0/moxifloxacin; 103060-53-3/Daptomycin; 165800-03-3/linezolid; 30604-81-0/polypyrrole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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