Document Detail


Polypyrrole-entrapped quinohemoprotein alcohol dehydrogenase. Evidence for direct electron transfer via conducting-polymer chains.
MedLine Citation:
PMID:  10464483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is reported for the first time that direct electron-transfer processes between a polypyrrole (PPY) entrapped quinohemoprotein alcohol dehydrogenase from Gluconobacter sp. 33 (QH-ADH) and a platinum electrode take place via the conducting-polymer network. The cooperative action of the enzyme-integrated prosthetic groups--pyrroloquinoline-quinone and hemes--is assumed to allow this electron-transfer pathway from the enzyme's active site to the conducting-polymer backbone. A hypothetical model of the electron transfer is proposed which is supported by the influence of various parameters, such as, e.g., ionic strength and nature of the buffer salts. This unusual electron-transfer pathway leads to an accentuated increase of the K M app value (102 mM) and hence to a significantly increased linear detection range of an ethanol sensor based on this enzyme.
Authors:
A Ramanavicius; K Habermuller; E Csöregi; V Laurinavicius; W Schuhmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-21     Completed Date:  1999-09-21     Revised Date:  2012-05-24    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3581-6     Citation Subset:  IM    
Affiliation:
Ruhr-Universität Bochum, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acetobacteraceae / enzymology
Alcohol Oxidoreductases*
Electron Transport
Ethanol
Models, Chemical
Models, Molecular
Platinum
Polymers*
Pyrroles*
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Polymers; 0/Pyrroles; 30604-81-0/polypyrrole; 64-17-5/Ethanol; 7440-06-4/Platinum; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.2.8/alcohol dehydrogenase (acceptor)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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