Document Detail


Polyphosphate stores enhance the ability of Vibrio cholerae to overcome environmental stresses in a low-phosphate environment.
MedLine Citation:
PMID:  16950899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vibrio cholerae, the causative agent of Asiatic cholera, has been reported to make large quantities of polyphosphate. Inorganic polyphosphate is a ubiquitous molecule with a variety of functions in prokaryotic and eukaryotic cells. We constructed a V. cholerae mutant with a deletion in the polyphosphate kinase (ppk) gene. The mutant was defective in polyphosphate biosynthesis. Deletion of ppk had no significant effect on production of cholera toxin, hemagglutinin/protease, motility, biofilm formation, and colonization of the suckling mouse intestine. The wild type and mutant had similar growth rates in rich and minimal medium and exhibited similar phosphate uptake and alkaline phosphatase induction. In contrast to ppk mutants from other gram-negative bacteria, the V. cholerae mutant survived prolonged starvation in LB medium and artificial seawater basal salts. The ppk mutant was significantly more sensitive to low pH, high salinity, and oxidative stress when it was cultured in low-phosphate minimal medium. The ppk mutant failed to induce catalase when it was downshifted to phosphorus-limiting conditions. Furthermore, the increased sensitivity of the ppk mutant to environmental stressors in phosphate-limited medium correlated with a diminished capacity to synthesize ATP from intracellular reservoirs. We concluded that polyphosphate protects V. cholerae from environmental stresses under phosphate limitation conditions. It has been proposed that toxigenic V. cholerae can survive in estuaries and brackish waters in which phosphorus and/or nitrogen can be a limiting nutrient. Thus, synthesis of large polyphosphate stores could enhance the ability of V. cholerae to survive in the aquatic environment.
Authors:
Iqbal K Jahid; Anisia J Silva; Jorge A Benitez
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-09-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-07     Completed Date:  2007-01-23     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7043-9     Citation Subset:  IM    
Affiliation:
Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr. SW, Atlanta, GA 30310-1495, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Suckling
Cholera / microbiology,  physiopathology
Culture Media
Gene Deletion
Heat-Shock Response*
Hydrogen-Ion Concentration
Mice
Oxidative Stress
Phosphates / metabolism*
Phosphotransferases (Phosphate Group Acceptor) / genetics,  metabolism
Polyphosphates / metabolism*
Sodium Chloride
Vibrio cholerae / genetics,  pathogenicity,  physiology*
Virulence
Grant Support
ID/Acronym/Agency:
AI063187/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Phosphates; 0/Polyphosphates; 7647-14-5/Sodium Chloride; EC 2.7.4.-/Phosphotransferases (Phosphate Group Acceptor); EC 2.7.4.1/polyphosphate kinase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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