Document Detail

Polyphosphate Deficiency Affects the Sliding Motility and Biofilm Formation of Mycobacterium smegmatis.
MedLine Citation:
PMID:  21882007     Owner:  NLM     Status:  Publisher    
Inorganic polyphosphate (polyP) is a ubiquitous linear polymer of hundreds of orthophosphate (Pi) residues linked by ATP-like, high-energy, phosphoanhydride bonds. The gene Rv1026 in Mycobacterium tuberculosis encodes a putative exopolyphosphatase which progressively hydrolyzes the terminal residues of polyP to liberate Pi. Rv1026 was cloned into the expressive plasmid pMV261. The resulting plasmid pRv1026 and the plasmid pMV261 were transformed into M. smegmatis strain mc(2)155 by electroporation. The recombinant M. smegmatis (pRv1026) showed relatively decreased polyP concentration and a phenotype different from the M. smegmatis (pMV261) in sliding motility and biofilm formation. The surfactant Tween 80 can enhance this effect on the sliding motility and biofilm formation of M. smegmatis. There are four different peaks between the gas chromatography of cellular wall fatty acid of the M. smegmatis (pRv1026) and the M. smegmatis (pMV261). These results indicate that polyP deficiency can affect the fatty acid composition of cellular wall and these alteration of cell wall might elucidate the reductive ability of strains to slide and form biofilm. This investigation provides novel recognition about the role of Rv1026, which provides novel clues for further study on the physiological role of Rv1026 in M. tuberculosis.
Tingyu Shi; Tiwei Fu; Jianping Xie
Related Documents :
11385057 - The influence of the albumin fraction on the bioavailability and postprandial utilizati...
9136117 - Influence of ovine oviducal amino acid concentrations and an ovine oestrus-associated g...
15660107 - A universal trend of amino acid gain and loss in protein evolution.
788717 - Adaptations with respect to salinity.
25169017 - Screening and molecular identifying overproducing gamma linolenic acid fungi and clonin...
25346517 - Osteoblast response to the surface of amino acid-functionalized hydroxyapatite.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-1
Journal Detail:
Title:  Current microbiology     Volume:  -     ISSN:  1432-0991     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing, 400715, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Brettanomyces as a Starter Culture in Rice-Steamed Sponge Cake: A Traditional Fermented Food in Chin...
Next Document:  Prevalence of Acanthamoeba from Tap Water in Rio Grande do Sul, Brazil.