| Polyphenol (-)-epigallocatechin gallate inhibits apoptosis induced by irradiation in human HaCaT keratinocytes. | |
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MedLine Citation:
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PMID: 15003511 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Green tea is a rich source of polyphenols, and (-)-epigallocatechin-3-gallate (EGCG) is a major constituent of green tea polyphenols. In the present study, we investigated the effect of EGCG on apoptosis induced by irradiation in the human keratinocytic cell line HaCaT. Irradiation by gamma-ray induced apoptosis with concomitant cleavage of caspase-3 and its in vivo substrate poly(ADP-ribose) polymerase. Treatment of cells with EGCG inhibited irradiation-induced apoptosis as detected by Hoechst staining and internucleosomal cleavage of DNA, and prevented the cleavage of these proteins by irradiation. We also found that the treatment of cells with EGCG alone suppressed cell growth and induced apoptosis in these cells. Our results suggest that EGCG inhibits irradiation-induced apoptosis by inactivating the caspase pathway in HaCaT cells. Our study also indicates that EGCG has a dual effect on the survival of these keratinocytes. |
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Authors:
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Hisayoshi Kondo; Sang-Hee Park; Keiko Watanabe; Yasuhiro Yamamoto; Makoto Akashi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 316 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-08 Completed Date: 2004-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 59-64 Citation Subset: IM |
Affiliation:
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Department of Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba 263-6555, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 Caspases / metabolism Catechin / analogs & derivatives*, pharmacology* Cell Division / drug effects Cell Line Humans Keratinocytes / drug effects*, metabolism, radiation effects Poly(ADP-ribose) Polymerases / metabolism |
| Chemical | |
Reg. No./Substance:
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154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
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