| Polyphenol-rich extracts from Solanum nigrum attenuated PKC alpha-mediated migration and invasion of hepatocellular carcinoma cells. | |
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MedLine Citation:
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PMID: 20349911 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Solanum nigrum L. (SN) has exhibited multiple biological effects such as anti-inflammation and antiproliferation. Protein kinase C (PKC) regulates cellular functions including proliferation, migration, and invasion. In the present investigation, we demonstrated that 12-o-tetradecanoylphobor-13-acetate (TPA) and constitutively activated PKC alpha significantly increased migration and invasion of HepG2 cells, while treatment with water or polyphenol extracts of SN (SNWE or SNPE) attenuated TPA-induced migration and invasion. SNWE and SNPE reduced TPA-elicited PKC alpha expression in a dose-dependent manner and obviously inhibited TPA-induced phosphorylation of p38 and ERK, respectively. Constitutively activated PKC alpha (caPKC alpha) significantly reversed the inhibitory effects of SNWE and SNPE on ERK and p38 activation. However, the antimigration effect of SNWE and SNPE could not be abrogated by caPKC alpha. Our results revealed the antimigration and anti-invasion effects of both extracts derived from SN, which may act as a promising therapeutic agent for the treatment of hepatocellular carcinoma. |
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Authors:
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Mon-Yuan Yang; Li-Sung Hsu; Chiung-Huei Peng; Yuan-Shong Shi; Cheng-Hsun Wu; Chau-Jong Wang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-05 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5806-14 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Biotechnology, Chung-Shan Medical University, Taichung, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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enzymology,
pathology* Cell Line, Tumor Flavonoids / pharmacology* Humans Liver Neoplasms / enzymology, pathology* Phenols / pharmacology* Phosphorylation Plant Extracts / pharmacology* Protein Kinase C-alpha / metabolism* Solanum nigrum / chemistry* Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/polyphenols; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.7.11.13/Protein Kinase C-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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