| Polypeptide-mediated switchable microarray of bacteria. | |
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MedLine Citation:
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PMID: 20355934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper describes a feasible solution for the bacterial cell death and contamination from cell division that occurs in microfluidic applications. The method adopts a smart thermoresponsive surface, highly resolved micropatterns, and surface-functionalized bacteria tagged with thermoresponsive molecules. We developed a method for controllable bacterial attachment and detachment using an elastin-like polypeptide (ELP). To create a smart surface with switchable properties, the surface of a glass substrate was conjugated with thermoresponsive ELP molecules. The attachment of bacterial cells to the ELP surface was induced by the hydrophobic affinity of the ELPs on the glass surface to tagged ELPs on the bacterial surface. A cell-repellent polymer was micropatterned to create a highly resolved space for specific bacterial adhesion. Reversible bacterial attachment and detachment was achieved by controlling the thermoresponsive phase transition of ELP molecules. Five different types of bacteria were successfully conjugated with ELPs and arrayed on the surface. The viability of the bacteria that had attached to the surface was evaluated by determining colony forming units of released bacteria on an agar plate. |
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Authors:
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Jonghwan Lee; Jaeyeon Jung; Kyunga Na; Pilwoo Heo; Jinho Hyun |
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Publication Detail:
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Type: Letter |
Journal Detail:
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Title: ACS applied materials & interfaces Volume: 1 ISSN: 1944-8244 ISO Abbreviation: ACS Appl Mater Interfaces Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2010-04-01 Completed Date: 2010-04-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101504991 Medline TA: ACS Appl Mater Interfaces Country: United States |
Other Details:
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Languages: eng Pagination: 1359-63 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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metabolism Bacteria / metabolism* Bacterial Adhesion Equipment Design Escherichia coli / metabolism Glass Materials Testing Oligonucleotide Array Sequence Analysis / methods Peptides / chemistry* Polymers / chemistry Surface Properties Temperature |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 0/Polymers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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