| Polypeptide amino acid composition and isoelectric point. II. Comparison between experiment and theory. | |
| | |
MedLine Citation:
|
PMID: 8678324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Experimental isoelectric points and amino acid compositional data for 58 proteins were compiled and organized. The experimental isoelectric points correlated well with the acidic to basic amino acid molar ratio. This agreement proved the usefulness of a recently presented analytical expression correlating explicitly protein isoelectric point to acid-base composition. Regressed acidic and basic dissociation constants were determined to be pK(alpha) = 4.9 and pK(beta) = 10.0, in fair agreement with the expected values of pK(alpha) = 4.2 and pK(beta) = 11.2. Theoretical isoelectric points determined by a more complete computational procedure were on the average in as good an agreement with the experimental values as those calculated via the theoretical approximation using the regressed dissociation constants. Thus, the analytical approximation is a powerful tool for the convenient and accurate calculation of protein isoelectric point from the amino acid composition. |
| | |
Authors:
|
C S Patrickios; E N Yamasaki |
Related Documents
:
|
16040024 - Comparative aspects of sperm membrane fatty acid composition in silver (vulpes vulpes) ... 4026864 - Sexual dimorphism in amino acid compositions of mouse prolactin. 12025874 - The classification of various organisms according to the free amino acid composition ch... 10760034 - Evolution of the hypervariable region of hepatitis c virus. 11086904 - Amino acids and peptides. xxxv. facile preparation of p-nitroanilide analogs by the sol... 2268354 - Substrate-dependent regulation of intracellular amino acid concentrations in cultured b... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Analytical biochemistry Volume: 231 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1995 Oct |
Date Detail:
|
Created Date: 1996-08-13 Completed Date: 1996-08-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 82-91 Citation Subset: IM |
Affiliation:
|
School of Chemistry and Molecular Sciences, University of Sussex, Falmer, Brighton, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis,
chemistry* Animals Humans Isoelectric Point Models, Theoretical Peptides / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: On-column reduction of catecholamine quinones in stainless steel columns during liquid chromatograph...
Next Document: Determination of local refractive index for protein and virus crystals in solution by Mach-Zehnder i...