Document Detail

Polypeptide amino acid composition and isoelectric point. II. Comparison between experiment and theory.
MedLine Citation:
PMID:  8678324     Owner:  NLM     Status:  MEDLINE    
Experimental isoelectric points and amino acid compositional data for 58 proteins were compiled and organized. The experimental isoelectric points correlated well with the acidic to basic amino acid molar ratio. This agreement proved the usefulness of a recently presented analytical expression correlating explicitly protein isoelectric point to acid-base composition. Regressed acidic and basic dissociation constants were determined to be pK(alpha) = 4.9 and pK(beta) = 10.0, in fair agreement with the expected values of pK(alpha) = 4.2 and pK(beta) = 11.2. Theoretical isoelectric points determined by a more complete computational procedure were on the average in as good an agreement with the experimental values as those calculated via the theoretical approximation using the regressed dissociation constants. Thus, the analytical approximation is a powerful tool for the convenient and accurate calculation of protein isoelectric point from the amino acid composition.
C S Patrickios; E N Yamasaki
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  231     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-08-13     Completed Date:  1996-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  82-91     Citation Subset:  IM    
School of Chemistry and Molecular Sciences, University of Sussex, Falmer, Brighton, United Kingdom.
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MeSH Terms
Amino Acids / analysis,  chemistry*
Isoelectric Point
Models, Theoretical
Peptides / chemistry*
Reg. No./Substance:
0/Amino Acids; 0/Peptides

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