Document Detail

Polyomavirus large- and small-T relieve middle-T-induced cell cycle arrest in normal fibroblasts.
MedLine Citation:
PMID:  10580053     Owner:  NLM     Status:  MEDLINE    
Papovavirus tumour antigens have been widely used to study cell growth regulation in cultured cells. We investigated the role of mouse polyomavirus T antigens, small-, middle- and large-T, in stimulating growth-arrested REF52 fibroblasts to enter the S phase. Microinjecting cells with cDNAs encoding the various T antigens showed: first, that middle-T expression blocked cell cycle stimulation by serum; second, that middle-T-arrested cells were released into the S phase upon coexpression of small-T; third, that expression of middle-T together with large-T committed resting cells to enter the cell cycle even in the absence of serum. Our data indicate that extensive cooperation among polyomavirus T antigens is essential for T antigen-mediated cell cycle stimulation in growth-arrested cells. In addition, the data suggest a new role for small-T in signalling to mitogenic pathways.
A Marti; K Ballmer-Hofer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general virology     Volume:  80 ( Pt 11)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-15     Completed Date:  1999-12-15     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2917-21     Citation Subset:  IM    
Institute of Medical Radiobiology at the Paul Scherrer Institute and of the University of Zürich, 5232- Villigen-PSI, Switzerland.
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MeSH Terms
Antigens, Polyomavirus Transforming / physiology*
Cell Cycle*
Fibroblasts / physiology
Retinoblastoma Protein / physiology
Tumor Suppressor Protein p53 / physiology
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Retinoblastoma Protein; 0/Tumor Suppressor Protein p53

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