| Polynucleotides XLVII. Synthesis and properties of poly(2-methylthio- and 2-ethylthioadenylic acid). Formation of non-Watson-Crick type complexes. | |
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MedLine Citation:
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PMID: 36915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly(2-methyl- and 2-ethylthioadenylic acid) were prepared by polymerization of corresponding diphosphates with Escherichia coli polynucleotide phosphorylase. These polynucleotides have relatively large hypochromicity of 30-35%. Acid titration of these polymers showed abrupt transition at pH 5.34-5.4, which may indicate that the introduction of alkylthio group at 2-position of adenine bases reduced their basicity. Thermal melting of these polymers showed no clear transition points at neutral pH, but in acidic media they have Tm values of 57 and 56 degrees C, somewhat lower than that of poly(A). Upon complex formation with poly(U), these poly(A) analogs showed only one poly(rs2A) . poly(U) type double-strand complexes, similar to that found in the case of poly(m2A) . poly(U). |
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Authors:
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T Fukui; M Ikehara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 562 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1979 May |
Date Detail:
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Created Date: 1979-09-17 Completed Date: 1979-09-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 527-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism Hot Temperature Hydrogen Bonding Hydrogen-Ion Concentration Nucleic Acid Conformation* Nucleic Acid Denaturation Poly A Poly U Polyribonucleotides* / chemical synthesis Spectrophotometry, Ultraviolet Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Polyribonucleotides; 24937-83-5/Poly A; 27416-86-0/Poly U |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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