| Polynucleotide helix geometry and stability. Spectroscopic, antigenic and interferon-inducing properties of deoxyribose-, ribose-, or 2'-deoxy-2'-fluororibose-containing duplexes of poly(inosinic acid) . poly(cytidylic acid). | |
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MedLine Citation:
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PMID: 6173383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circular dichroism, absorbance temperature profiles, antigenic properties, and interferon-inducing capacity of the nine double-stranded complexes between poly(inosinic acid) and poly(cytidylic acid) [(rI)n . (rC)n] and their deoxy- and 2'-deoxy-2'-fluoro-analogues were studied. The complexes containing only ribose or 2'-fluororibose chains showed similar CD spectra and thermal stabilities. Anti-(rI)n . (rC)n antibodies were well recognized by the duplexes containing 2'-fluororibose in either one strand. These two duplexes were also efficient interferon inducers. Presence of fluororibose in both strands decreased the affinity of anti-(rI)n . (rC)n antibodies slightly and abolished interferon-inducing activity. All polydeoxyriboside-containing complexes showed CD spectra significantly different from the previous group; they showed also 40- to 100-times lower affinity for the anti-(rI)n . (rC)n antibodies, and did not induce interferon. It is concluded that the structure of (rI)n . (rC)n, an A-type helix, is little perturbed by substitution of one or both strands by 2'-fluororibose. Substitution by deoxyribose in either strand considerably changes the structure of the helices. The hybrids containing poly(deoxycytidylic acid) retain at least some of the structural features of the B-type helix poly(deoxyinosinic acid) . poly(deoxycytidylic acid). |
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Authors:
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N Kakiuchi; C Marck; N Rousseau; M Leng; E De Clerq; W Guschlbauer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Feb |
Date Detail:
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Created Date: 1982-04-12 Completed Date: 1982-04-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1924-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Circular Dichroism Deoxyribose / analogs & derivatives* Epitopes / analysis Immunodiffusion Interferons / biosynthesis Kidney / drug effects Nucleic Acid Conformation Nucleic Acid Denaturation Poly I / immunology, pharmacology Poly I-C* / immunology, pharmacology Rabbits Radioimmunoassay Spectrophotometry, Ultraviolet Structure-Activity Relationship Temperature |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 24939-03-5/Poly I-C; 25249-22-3/Poly I; 533-67-5/Deoxyribose; 7226-33-7/2'-deoxy-2'-fluororibose; 9008-11-1/Interferons |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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