| Polymorphonuclear leukocyte membrane fluidity and cytosolic Ca2+ content in different clinical conditions. | |
| | |
MedLine Citation:
|
PMID: 9356786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of the study was to evaluate the polymorphonuclear leukocyte (PMN) membrane fluidity and PMN cytosolic Ca2+ content in several clinical conditions: diabetes mellitus, vascular atherosclerotic disease (VAD), chronic renal failure (CRF), essential hypertension (EH). In 13 subjects with insulin-dependent diabetes mellitus (IDDM), in 24 subjects with non-insulin-dependent diabetes mellitus (NIDDM), in 42 VAD subjects, in 23 VAD subjects with NIDDM, in 15 subjects with CRF and in 12 subjects with EH, we determined the PMN membrane fluidity, obtained marking unstimulated PMN cells with fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), and considering the fluorescence polarization degree, and the PMN cytosolic Ca2+ content, obtained marking unstimulated PMN cells with the fluorescent probe Fura2-AM and considering the ratio between the Fura2-Ca2+ complex and the unchelated Fura 2 fluorescence intensity. From the obtained data it is evident that PMN membrane fluidity does not distinguish normals from IDDM subjects, NIDDM subjects, VAD subjects with and without NIDDM, CRF subjects and hypertensives. PMN cytosolic Ca2+ content, in comparison with normal controls, is significantly increased in VAD subjects (p < 0.01), in VAD subjects with NIDDM (p < 0.001), in CRF subjects (p < 0.001) and in hypertensives (p < 0.05). No correlation was found between PMN membrane fluidity and PMN cytosolic Ca2+ content. The study of these PMN parameters can be useful in the understanding of the role of leukocytes in the vascular damage that characterizes these clinical conditions. |
| | |
Authors:
|
G Caimi; B Canino; M Montana; G Ventimiglia; A Catania; R Lo Presti |
Related Documents
:
|
9330586 - Vitamin e and nicotinamide have similar effects in maintaining residual beta cell funct... 11665396 - Intracrevicular application of 0.3% flurbiprofen gel and 0.3% triclosan gel as anti inf... 9702436 - Hydroperoxides in plasma are reduced by intensified insulin treatment. a randomized con... 3678566 - Salivary flow rate and salivary glucose concentration in patients with diabetes mellitu... 16631806 - Long-term efficacy of sulfonylureas: a united kingdom prospective diabetes study perspe... 7383946 - Dermatitis herpetiformis and diabetes mellitus. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Clinical hemorheology and microcirculation Volume: 17 ISSN: 1386-0291 ISO Abbreviation: Clin. Hemorheol. Microcirc. Publication Date: 1997 May-Jun |
Date Detail:
|
Created Date: 1997-12-18 Completed Date: 1997-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9709206 Medline TA: Clin Hemorheol Microcirc Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 217-23 Citation Subset: IM |
Affiliation:
|
Istituto di Clinica Medica e Malattie Cardiovascolari, Università di Palermo, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Aged Arteriosclerosis / blood Calcium / metabolism* Case-Control Studies Cytosol / metabolism* Diabetes Mellitus, Type 1 / blood Diabetes Mellitus, Type 2 / blood Female Humans Kidney Failure, Chronic / blood Male Membrane Fluidity* Middle Aged Neutrophils / metabolism* |
| Chemical | |
Reg. No./Substance:
|
7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Variations of erythrocyte morphology in different pathologies.
Next Document: Evaluation of the success of hemodilution therapy for fetal growth retardation by Doppler sonography...