Document Detail


Polymorphonuclear leukocyte heterogeneity in neonates and adults.
MedLine Citation:
PMID:  3719097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used a mouse monoclonal antibody (31D8) to determine whether differences in neutrophil (PMN) subpopulations might help explain decreased PMN chemotaxis in neonates compared with that of adults. 31D8 has been shown to bind heterogeneously to adult PMNs. Approximately 80% of the PMNs that strongly bind 31D8 (31D8 "bright") are the same cells that depolarize and migrate chemotactically when stimulated with the chemoattractant N-formyl-methionylleucylphenylalanine, while the 20% that weakly bind 31D8 fail to similarly respond. All neonatal PMNs bound 31D8 heterogeneously. There was a smaller population of 31D8 "bright" cells in neonates at birth (76% +/- 6%, n = 45) compared with that of neonates at three to 15 days of age (82% +/- 5%, n = 10, P less than 0.002) and both were smaller than that of adults (88% +/- 4%, n = 45, P less than 0.001 and P less than 0.001). Neonatal cord PMNs, which traversed a micropore filter in a modified Boyden chemotaxis chamber in the presence of a chemoattractant, had an increased percentage of 31D8 "bright" cells (89% +/- 7%) than did PMNs which remained above the filter (82% +/- 7%, n = 10, P = 0.034). PMN chemotaxis was less in neonates at birth (32.7 +/- 4.5 micron) than at three to six days of age (36.8 +/- 11.3 micron) and both were decreased compared with that of adults (69.1 +/- 12.4 micron, P less than 0.001 and P less than 0.001). These findings indicate that decreased PMN chemotaxis in neonates may be due in part to a smaller PMN subpopulation of highly motile cells.
Authors:
P J Krause; H L Malech; J Kristie; C M Kosciol; V C Herson; L Eisenfeld; W T Pastuszak; A Kraus; B Seligmann
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  68     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-07-30     Completed Date:  1986-07-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  200-4     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Age Factors
Antibodies, Monoclonal / diagnostic use
Binding, Competitive
Chemotaxis, Leukocyte* / drug effects
Humans
Infant, Newborn
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Neutrophils / immunology*
Grant Support
ID/Acronym/Agency:
AI 19768/AI/NIAID NIH HHS; CA 08341/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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