Document Detail


Polymorphisms of the SAMHD1 gene are not associated with the infection and natural control of HIV type 1 in Europeans and African-Americans.
MedLine Citation:
PMID:  22530776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HIV-1 restriction factor SAM domain and HD domain-containing protein 1 (SAMHD1) blocks HIV-1 infection in human myeloid cells. Mutations in the SAMHD1 gene are associated with rare genetic diseases including Aicardi-Goutieres syndrome. However, it is unknown whether polymorphisms of SAMHD1 are associated with infection and natural control of HIV-1 in humans. Our objective was to determine whether the expression of SAMHD1 mRNA is affected by common single nucleotide polymorphisms (SNPs) in SAMHD1 and whether the SNPs are associated with HIV-1 infection status. Using a tagging SNP approach, we determined the association between eight tagging SNPs in SAMHD1 and the mRNA expression in B-lymphocyte cell lines from 70 healthy white donors. We identified one SNP (rs1291142) that was significantly associated with SAMHD1 mRNA expression, with minor allele carriers having 30% less mRNA levels (p=0.015). However, after analyzing the published genome-wide association study data of 857 HIV-1 controllers and 2088 HIV-1 progressors from the European and African-American cohorts, we did not find a significant association between SNPs in SAMHD1 and HIV-1 infection status, including SNP rs1291142 (p>0.05). We also observed 2- to 6-fold variations of SAMHD1 mRNA levels in primary B-lymphocytes, CD4(+) T-lymphocytes, and CD14(+) monocytes from five healthy donors. Our results suggest that common regulatory polymorphism(s) exist in the SAMHD1 gene that affects its mRNA expression in B-lymphocyte cell lines from healthy whites. However, polymorphisms of SAMHD1 are unlikely to contribute to the infection and natural control of HIV-1 in European and African-American individuals.
Authors:
Sirena Coon; Danxin Wang; Li Wu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-01
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  28     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-22     Completed Date:  2013-04-23     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1565-73     Citation Subset:  IM; X    
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MeSH Terms
Descriptor/Qualifier:
African Americans
B-Lymphocytes / immunology
European Continental Ancestry Group
Gene Expression Profiling
Genetic Predisposition to Disease*
HIV Infections / genetics*,  immunology*
Humans
Monomeric GTP-Binding Proteins / genetics*
Polymorphism, Single Nucleotide*
Grant Support
ID/Acronym/Agency:
AI078762/AI/NIAID NIH HHS; AI098524/AI/NIAID NIH HHS; GM092655/GM/NIGMS NIH HHS; R21 AI098524/AI/NIAID NIH HHS; R21 AI102822/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/SAMHD1 protein, human; EC 3.6.5.2/Monomeric GTP-Binding Proteins
Comments/Corrections

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