Document Detail


Polymorphism of the thrombostasin gene in the horn fly (Haematobia irritans) revealed in a cDNA library and in genomic DNA.
MedLine Citation:
PMID:  11683272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thrombostasin (TS) is a newly described thrombin-inhibiting protein isolated from the saliva of the horn fly (Haematobia irritans), a blood-sucking ectoparasite of cattle. This report provides a detailed characterization of the TS gene and the first analysis of the allelic complexity of a gene for an anti-hemostatic protein from a blood-feeding insect. Multiple point mutations at fixed positions in the TS gene were identified in a cDNA library prepared from mRNA isolated from horn fly salivary glands. When translated, the variant mRNAs would specify five biochemically active peptides that differ in molecular weight, isoelectric point and predicted secondary structure. Allelic variation with the same mutation pattern was revealed in the genomes of individual flies collected in the field and sampled from a long-standing laboratory colony. Approximately 60% of flies examined carried heterozygous alleles, including five additional alleles not found in the cDNA library. Comparative analysis of the allelic mutations and the predicted effects on secondary structures of the active proteins produced suggest that the TS gene may be undergoing evolutionary selection.
Authors:
D Zhang; M S Cupp; E W Cupp
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  266     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  296-302     Citation Subset:  IM    
Affiliation:
Department of Entomology and Plant Pathology, Auburn University, AL 36849-5413, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
DNA / chemistry,  genetics*
DNA, Complementary / chemistry,  genetics*
Female
Gene Library
Insect Proteins / genetics*
Male
Molecular Sequence Data
Muscidae / genetics*
Polymorphism, Genetic
Protein Isoforms / genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Insect Proteins; 0/Protein Isoforms; 0/Thrombostasin protein, Haematobia irritans; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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