Document Detail


Polymorphism of Saccharomyces cerevisiae aquaporins.
MedLine Citation:
PMID:  10870101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aquaporin water channels facilitate the transmembrane diffusion of water and higher organisms possess a large number of isoforms. The genome of the yeast Saccharomyces cerevisiae contains two highly similar aquaporin genes, AQY1 and AQY2. AQY1 has been shown to encode a functional water channel but only in certain laboratory strains. Here we show that the AQY2 gene is interrupted by an 11 bp deletion in 23 of the 27 laboratory strains tested, with the exception of strains from the sigma 1278b background, which also exhibit a functional Aqy1p. However, although the AQY2 gene from sigma 1278b is highly homologous to functional aquaporins, we did not observe Aqy2p-mediated water transport in Xenopus oocytes. A survey of 52 yeast strains revealed that all industrial and wild yeasts carry the allele encoding a functional Aqy1p, while none of these strains appear to have a functional Aqy2p. We conclude that natural and industrial conditions provide selective pressure to maintain AQY1 but apparently not AQY2.
Authors:
V Laizé; F Tacnet; P Ripoche; S Hohmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  16     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-10-05     Completed Date:  2000-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  897-903     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Affiliation:
Department of Cell and Molecular Biology, Microbiology, Göteborg University, Box 462, SE-40530 Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Aquaporins / chemistry,  genetics*,  metabolism
Base Sequence
Fungal Proteins / chemistry,  genetics,  metabolism
Molecular Sequence Data
Polymorphism, Genetic*
Restriction Mapping
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins*
Sequence Alignment
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/AQY1 protein, S cerevisiae; 0/AQY2 protein, S cerevisiae; 0/Aquaporins; 0/Fungal Proteins; 0/Saccharomyces cerevisiae Proteins

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