| Polymorphism of F-actin assembly. 1. A quantitative phase diagram of F-actin. | |
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MedLine Citation:
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PMID: 8611509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have made the first quantitative phase diagram of actin filament (F-actin) assembly represented by the concentration of F-actin and the chi parameter which characterizes solvent-solute interaction energy. We manipulated the chi value of F-actin by adding a high molecular weight poly- (ethylene glycol) with average molecular weight 6000 (PEG 6K). The preferential exclusion of PEG 6K from the region adjacent to F-actin increases the chi value of F-actin. We qualified the PEG 6K-induced increase of the chi value through analysis of the PEG-induced solubility change of protein. The phase diagram shows that F-actin changes its assembly structure from isotropic disordered distribution to anisotropic ordered phase of a lyotropic liquid crystalline with an increase in the concentration and to concentrated anisotropic ordered phase of a crystalline-like bundle with a small increase in chi respectively, in the physiological concentration range. The formation of the crystalline- like bundle suggests that some specific force may act between F-actin. The present results demonstrate that F-actin can take various assembly structures as observed in cytoplasm by itself, indicating that the versatility of F-actin assembly in cytoplasm may be based on the thermodynamic properties of F-actin as a rod-like molecule. |
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Authors:
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A Suzuki; M Yamazaki; T Ito |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 35 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-06-06 Completed Date: 1996-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5238-44 Citation Subset: IM |
Affiliation:
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Department of Biophysics, Kyoto University School of Science, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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chemistry*,
drug effects,
ultrastructure Crystallization Models, Chemical Polyethylene Glycols / pharmacology Solubility Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Polyethylene Glycols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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