Document Detail

Polymorphism of F-actin assembly. 1. A quantitative phase diagram of F-actin.
MedLine Citation:
PMID:  8611509     Owner:  NLM     Status:  MEDLINE    
We have made the first quantitative phase diagram of actin filament (F-actin) assembly represented by the concentration of F-actin and the chi parameter which characterizes solvent-solute interaction energy. We manipulated the chi value of F-actin by adding a high molecular weight poly- (ethylene glycol) with average molecular weight 6000 (PEG 6K). The preferential exclusion of PEG 6K from the region adjacent to F-actin increases the chi value of F-actin. We qualified the PEG 6K-induced increase of the chi value through analysis of the PEG-induced solubility change of protein. The phase diagram shows that F-actin changes its assembly structure from isotropic disordered distribution to anisotropic ordered phase of a lyotropic liquid crystalline with an increase in the concentration and to concentrated anisotropic ordered phase of a crystalline-like bundle with a small increase in chi respectively, in the physiological concentration range. The formation of the crystalline- like bundle suggests that some specific force may act between F-actin. The present results demonstrate that F-actin can take various assembly structures as observed in cytoplasm by itself, indicating that the versatility of F-actin assembly in cytoplasm may be based on the thermodynamic properties of F-actin as a rod-like molecule.
A Suzuki; M Yamazaki; T Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  35     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-06-06     Completed Date:  1996-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5238-44     Citation Subset:  IM    
Department of Biophysics, Kyoto University School of Science, Japan.
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MeSH Terms
Actins / chemistry*,  drug effects,  ultrastructure
Models, Chemical
Polyethylene Glycols / pharmacology
Reg. No./Substance:
0/Actins; 0/Polyethylene Glycols

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