| Polymerized placenta hemoglobin attenuates ischemia/reperfusion injury and restores the nitroso-redox balance in isolated rat heart. | |
| | |
MedLine Citation:
|
PMID: 19038330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ischemia/reperfusion (I/R) injury mainly caused by oxidative stress plays a major role in cardiac damage. The extent of the I/R injury is also an important factor that determines the function of a transplanted heart. This study first examined whether hemoglobin-based oxygen carriers (HBOCs) could protect isolated rat heart from I/R injury and then elucidated the underlying mechanism. Using the Langendorff model, isolated Sprague-Dawley rat hearts were arrested and stored at 4 degrees C for 8 h and then reperfused for 2 h. Compared with St. Thomas' solution (STS) and rat self blood in STS, polymerized placenta hemoglobin (PolyPHb) in STS greatly improved heart contraction and decreased infarction size. The extent of myocardial apoptosis was also significantly decreased, which was related to reduced iNOS-derived nitric oxide production, increased protein ratio of Bcl-2/Bax, and reduced caspase-3 activity and cleavage level. Furthermore, PolyPHb in STS did not increase malondialdehyde, peroxynitrite, or mitochondrial hydrogen peroxide formation, but greatly elevated superoxide dismutase activity and preserved mitochondrial ATP synthesis, which served to maintain redox homeostasis in I/R heart. In conclusion, our results demonstrate that HBOCs protected isolated heart from I/R injury and this protection was associated with attenuation of NO-mediated myocardial apoptosis and restoration of the nitroso-redox balance. |
| | |
Authors:
|
Tao Li; Jing Li; Jin Liu; Pu Zhang; Wei Wu; Ronghua Zhou; Guohua Li; Wensheng Zhang; Mingliang Yi; Han Huang |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-06 |
Journal Detail:
|
Title: Free radical biology & medicine Volume: 46 ISSN: 1873-4596 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-01-09 Completed Date: 2009-09-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
|
Languages: eng Pagination: 397-405 Citation Subset: IM |
Affiliation:
|
Laboratory of Anesthesiology and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis Female Heart Function Tests Heart Transplantation Hemoglobins* Humans Hydrogen Peroxide / metabolism Male Mitochondria, Heart / metabolism*, ultrastructure Myocardium / enzymology*, ultrastructure Nitrites / analysis Organ Preservation Solutions* Oxidation-Reduction Oxidative Stress Placenta* Pregnancy Pyridoxal Phosphate / analogs & derivatives* Rats Rats, Sprague-Dawley Reperfusion Injury / prevention & control Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Hemoglobins; 0/Nitrites; 0/Organ Preservation Solutions; 0/polyhemoglobin-pyridoxal-5-phosphate; 54-47-7/Pyridoxal Phosphate; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increase in mitochondrial biogenesis, oxidative stress, and glycolysis in murine lymphomas.
Next Document: Oxidative stress induces macroautophagy of amyloid beta-protein and ensuing apoptosis.