Document Detail


Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of bacterial community structure in the food, intestines, and feces of earthworms.
MedLine Citation:
PMID:  21887635     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The bacterial communities in the food, intestines, and feces of earthworms were investigated by PCR-denaturing Gradient gel electrophoresis (DGGE). In this study, PCR-DGGE was optimized by testing 6 universal primer sets for microbial 16S rRNA in 6 pure culture strains of intestinal microbes in earthworms. One primer set effectively amplified 16S rRNA from bacterial populations that were found in the food, intestines, and feces of earthworms. Compared with the reference markers from the pure culture strains, the resulting DGGE profiles contained 28 unique DNA fragments. The dominant microorganisms in the food, intestines, and feces of earthworms included Rhodobacterales bacterium, Fusobacteria, Ferrimonas marina, Aeromonas popoffii, and soil bacteria. Other straisn, such as Acinetobacter, Clostridium, and Veillonella, as well as rumen bacteria and uncultured bacteria also were present. These results demonstrated that PCR-DGGE analysis can be used to elucidate bacterial diversity and identify unculturable microorganisms.
Authors:
Sung Wook Hong; Ju Sam Lee; Kun Sub Chung
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Publication Detail:
Type:  Journal Article     Date:  2011-09-02
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  49     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-09-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  544-50     Citation Subset:  IM    
Affiliation:
Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea.
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