Document Detail


Polymerase chain reaction : basic protocols.
MedLine Citation:
PMID:  21400259     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The melding of a technique for repeated rounds of DNA synthesis with the discovery of a thermostable DNA polymerase has given scientists the very powerful technique known as polymerase chain reaction (PCR). PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers. These reactions may be carried out with any DNA polymerase and result in the synthesis of defined portions of the original DNA sequence. However, in order to achieve more than one round of synthesis, the templates must again be denatured, which requires temperatures well above those that inactivate most enzymes. Therefore, initial attempts at cyclic DNA synthesis were carried out by adding fresh polymerase after each denaturation step (1,2). The cost of such a protocol becomes rapidly prohibitive.
Authors:
B C Delidow; J P Lynch; J J Peluso; B A White
Related Documents :
21487389 - Sld7, an sld3-associated protein required for efficient chromosomal dna replication in ...
14748709 - Single-molecule studies of dna and rna four-way junctions.
21374199 - Bacterial transformation (kushner method).
21125329 - Molecular diagnosis of francisellosis, a systemic granulomatous inflammatory disease in...
21484129 - Influence of serum hbv dna load on recurrence of hepatocellular carcinoma after treatme...
21173199 - Bidentate rna-magnesium clamps: on the origin of the special role of magnesium in rna f...
9372939 - Dna in transcriptionally silent chromatin assumes a distinct topology that is sensitive...
19445949 - Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombina...
14736459 - Evidence for high specificity and efficiency of multiple recombination signals in mixed...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  15     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  1993  
Date Detail:
Created Date:  2011-03-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-29     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Connecticut Health Center, Farmington, CT.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sacral neuromodulation in patients with multiple sclerosis.
Next Document:  Selection of primers for polymerase chain reaction.