| Polymerase-chain-reaction-based detection of fetal rhesus D and Y-chromosome-specific DNA in the whole blood of pregnant women during different trimesters of pregnancy. | |
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MedLine Citation:
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PMID: 17709918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to determine whether or not a noninvasive procedure utilizing maternal peripheral blood as the source of DNA and polymerase chain reaction (PCR) could be used to detect fetal rhesus D (RhD) status as well as fetal gender during different gestational stages of pregnancy. MATERIALS AND METHODS: Maternal blood samples were obtained from 54 RhD-negative pregnant women during the first trimester (6-13 weeks, n = 14), second trimester (14-26 weeks, n = 26) and third trimester (27-40 weeks, n = 14). Genomic DNA was extracted from the whole blood and analyzed by seminested and nested PCR for detection of DNA sequences corresponding to RhD (n = 54) and Y chromosome (n = 48) using RhD and Y-chromosome-specific oligonucleotide primers, respectively. The seminested/nested PCR results were compared with the RhD status and gender of the babies after delivery. RESULTS: The sensitivity and specificity of seminested PCR for detection of fetal RhD positivity in whole blood of pregnant women were 81 and 100%, respectively, while the sensitivity and specificity of nested PCR for detection of male fetuses, using Y-chromosome-specific DNA as a marker, were 96 and 91%, respectively. There were no significant differences in the PCR results with samples obtained from women at different gestational stages of pregnancy. CONCLUSION: Seminested and nested PCRs for detection of fetal RhD and gender status, respectively, by using the blood of pregnant women during different gestational stages of pregnancy, are reliable noninvasive procedures with high sensitivity and specificity. |
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Authors:
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M K Al-Yatama; A S Mustafa; F M Al-Kandari; N Khaja; K Zohra; R A Monem; S Abraham |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Medical principles and practice : international journal of the Kuwait University, Health Science Centre Volume: 16 ISSN: 1423-0151 ISO Abbreviation: Med Princ Pract Publication Date: 2007 |
Date Detail:
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Created Date: 2007-08-21 Completed Date: 2007-11-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8901334 Medline TA: Med Princ Pract Country: Switzerland |
Other Details:
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Languages: eng Pagination: 327-32 Citation Subset: IM |
Copyright Information:
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2007 S. Karger AG, Basel |
Affiliation:
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Department of Obstetrics and Gynecology, Faculty of Medicine, Kuwait University, Kuwait. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Human, Y* DNA / blood* Female Fetal Blood / chemistry* Gestational Age Humans Male Polymerase Chain Reaction / methods* Pregnancy Pregnancy Trimesters / blood* Prenatal Diagnosis / methods* Rh Isoimmunization / blood, genetics Rh-Hr Blood-Group System / blood*, genetics Sensitivity and Specificity Sex Determination (Analysis) / methods |
| Chemical | |
Reg. No./Substance:
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0/Rh-Hr Blood-Group System; 0/Rho(D) antigen; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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