Document Detail


Polymerase chain reaction amplification of human papillomavirus DNA from archival, Papanicolaou-stained cervical smears.
MedLine Citation:
PMID:  8669167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To test the applicability of four protocols in recovering DNA suitable for amplification with the polymerase chain reaction (PCR) in archival, Papanicolaou-stained cervical smears. STUDY DESIGN: The most efficient method was used to isolate DNA from 11 archival, Papanicolaou-stained smears with cytopathic changes due to human papillomavirus (HPV) infection to confirm the presence of HPV DNA. RESULTS: beta-Globin was successfully amplified in all smears, while HPV DNA was detected in 6 of 11. Four of the four HPV DNA-negative smears were classified as high grade squamous intraepithelial lesions. Failure to detect HPV DNA might have been due to the low copy number of HPV DNA or deletion of the L1 region. CONCLUSION: High cellularity and the method of recovering DNA from the smear are important determinants of successful amplification of HPV DNA in archival cervical smears.
Authors:
M Puranen; S Saarikoski; K Syrjänen; S Syrjänen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cytologica     Volume:  40     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    1996 May-Jun
Date Detail:
Created Date:  1996-08-05     Completed Date:  1996-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  391-5     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Kuopio, Finland.
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MeSH Terms
Descriptor/Qualifier:
Beta-Globulins / genetics
Carcinoma, Squamous Cell / genetics,  pathology,  virology
DNA Primers
DNA, Viral / genetics,  isolation & purification
Electrophoresis, Agar Gel
Female
Gene Amplification
Humans
Papillomaviridae / genetics*,  isolation & purification
Polymerase Chain Reaction / methods*
Vaginal Smears / methods*
Chemical
Reg. No./Substance:
0/Beta-Globulins; 0/DNA Primers; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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