Document Detail


Polymerase chain reaction¿based assays for the diagnosis of human brucellosis.
MedLine Citation:
PMID:  25082566     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed.
Authors:
Ying Wang; Zhanli Wang; Yaxian Zhang; Liyun Bai; Yue Zhao; Chunfang Liu; An Ma; Hui Yu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-1
Journal Detail:
Title:  Annals of clinical microbiology and antimicrobials     Volume:  13     ISSN:  1476-0711     ISO Abbreviation:  Ann. Clin. Microbiol. Antimicrob.     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101152152     Medline TA:  Ann Clin Microbiol Antimicrob     Country:  -    
Other Details:
Languages:  ENG     Pagination:  31     Citation Subset:  -    
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