| Polymer brush-stabilized polyplex for a siRNA carrier with long circulatory half-life. | |
| | |
MedLine Citation:
|
PMID: 17583369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Delivery systems of small interfering RNA (siRNA) are the key to siRNA therapeutic application. In this study, we prepared and evaluated a series of cationic comb-type copolymers (CCCs) possessing a polycationic backbone (less than 30 weight (wt) %) and abundant water-soluble side chains (more than 70 wt.%) as a siRNA carrier with prolonged blood circulation time. Markedly, the CCC with the higher side chain content (10 wt.% PLL and 90 wt.% PEG) showed stronger interaction with siRNA than that with the lower content (30 wt.% PLL and 70 wt.% PEG), suggesting that highly dense PEG brush reinforces interpolyelectrolyte complex between the PLL backbone and siRNA. The siRNA complexed with the CCC was resistant to nucleases in 90% plasma for 24 h in vitro. The CCC having the higher side chain content increased circulation time of siRNA in mouse bloodstream by 100-fold. Surprisingly, even when the CCC and siRNA were separately injected into mouse at 20 min interval, blood circulation of post-injected siRNA was significantly increased. These results imply that the CCC has higher selectivity in its ionic interaction with siRNA than other anionic substances in blood stream. To our knowledge, this is the first example of a polyplex carrier that prolongs blood circulation time of unmodified siRNA without resource-consuming preparation process. |
| | |
Authors:
|
Ayumi Sato; Sung Won Choi; Miwa Hirai; Asako Yamayoshi; Rui Moriyama; Takeshi Yamano; Motoki Takagi; Arihiro Kano; Akira Shimamoto; Atsushi Maruyama |
Related Documents
:
|
10534759 - Protein a tangential flow affinity membrane cartridge for extracorporeal immunoadsorpti... 12881609 - Collateral circulation. 19867949 - Studies on the circulation in man : xvi. a study of the development of the collateral c... 451829 - Cardiovascular effects of methyl methacrylate monomer. 92339 - The relation between the concentration of placental specific proteins in retroplacental... 7560739 - Dermal absorption of polycyclic aromatic hydrocarbons in the blood-perfused pig ear. 8325179 - Superior mesenteric artery blood flow in celiac disease. 7107229 - Effect of nonsteroidal anti-inflammatory agents on immune complex- and chemotactic fact... 2332369 - Eperythrozoon wenyonii infection in dairy cattle. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-03 |
Journal Detail:
|
Title: Journal of controlled release : official journal of the Controlled Release Society Volume: 122 ISSN: 1873-4995 ISO Abbreviation: J Control Release Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-09-21 Completed Date: 2007-11-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8607908 Medline TA: J Control Release Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 209-16 Citation Subset: IM |
Affiliation:
|
GeneCare Research Institute Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-0063, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Dextrans / chemistry* Drug Carriers / chemistry* Drug Stability Half-Life Injections, Intravenous Male Mice Mice, Inbred ICR Polyethylene Glycols / chemistry* Polylysine / analogs & derivatives*, chemistry RNA, Small Interfering / administration & dosage*, blood, pharmacokinetics* Solubility |
| Chemical | |
Reg. No./Substance:
|
0/Drug Carriers; 0/Polyethylene Glycols; 0/RNA, Small Interfering; 0/poly(lysine)-graft-dextran; 0/polylysine-graft-(poly(ethylene glycol)); 25104-18-1/Polylysine; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The identification and characterization of a monoclonal antibody to the vaccinia virus E3 protein.
Next Document: Nuclear functions of endocytic proteins.