Document Detail


Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2.
MedLine Citation:
PMID:  8679107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3-trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9-octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic-liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.
Authors:
K D Mack; R L Walzem; K Lehmann-Bruinsma; J S Powell; J B Zeldis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  23 ( Pt 3)     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-22     Completed Date:  1996-08-22     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  217-20     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of California, Davis 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Cations
Electrophoresis, Agar Gel
Fatty Acids, Monounsaturated / chemistry,  metabolism
Hepatoblastoma / pathology*
Humans
Liposomes
Liver Neoplasms / pathology*
Luciferases / metabolism
Particle Size
Phosphatidylethanolamines / chemistry,  metabolism
Plasmids / metabolism
Polylysine / metabolism,  pharmacology*
Quaternary Ammonium Compounds / chemistry,  metabolism
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/1-2-di-(9-octadecenoyl)-sn-glycero-3-phosphoethethanolamine; 0/Cations; 0/Fatty Acids, Monounsaturated; 0/Liposomes; 0/Phosphatidylethanolamines; 0/Quaternary Ammonium Compounds; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 25104-18-1/Polylysine; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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