| Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2. | |
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MedLine Citation:
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PMID: 8679107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3-trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9-octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic-liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells. |
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Authors:
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K D Mack; R L Walzem; K Lehmann-Bruinsma; J S Powell; J B Zeldis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 23 ( Pt 3) ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-22 Completed Date: 1996-08-22 Revised Date: 2007-03-21 |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 217-20 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of California, Davis 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cations Electrophoresis, Agar Gel Fatty Acids, Monounsaturated / chemistry, metabolism Hepatoblastoma / pathology* Humans Liposomes Liver Neoplasms / pathology* Luciferases / metabolism Particle Size Phosphatidylethanolamines / chemistry, metabolism Plasmids / metabolism Polylysine / metabolism, pharmacology* Quaternary Ammonium Compounds / chemistry, metabolism Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/1-2-di-(9-octadecenoyl)-sn-glycero-3-phosphoethethanolamine; 0/Cations; 0/Fatty Acids, Monounsaturated; 0/Liposomes; 0/Phosphatidylethanolamines; 0/Quaternary Ammonium Compounds; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 25104-18-1/Polylysine; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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