| Polyhydroxyalkanoate copolymers from forest biomass. | |
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MedLine Citation:
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PMID: 16761168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potential for the use of woody biomass in poly-beta-hydroxyalkanoate (PHA) biosynthesis is reviewed. Based on previously cited work indicating incorporation of xylose or levulinic acid (LA) into PHAs by several bacterial strains, we have initiated a study for exploring bioconversion of forest resources to technically relevant copolymers. Initially, PHA was synthesized in shake-flask cultures of Burkholderia cepacia grown on 2.2% (w/v) xylose, periodically amended with varying concentrations of levulinic acid [0.07-0.67% (w/v)]. Yields of poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) [P(3HB-co-3HV)] from 1.3 to 4.2 g/l were obtained and could be modulated to contain from 1.0 to 61 mol% 3-hydroxyvalerate (3HV), as determined by 1H and 13C NMR analyses. No evidence for either the 3HB or 4HV monomers was found. Characterization of these P(3HB-co-3HV) samples, which ranged in molecular mass (viscometric, Mv) from 511-919 kDa, by differential scanning calorimetry and thermogravimetric analyses (TGA) provided data which were in agreement for previously reported P(3HB-co-3HV) copolymers. For these samples, it was noted that melting temperature (Tm) and glass transition temperature (Tg) decreased as a function of 3HVcontent, with Tm demonstrating a pseudoeutectic profile as a function of mol% 3HV content. In order to extend these findings to the use of hemicellulosic process streams as an inexpensive carbon source, a detoxification procedure involving sequential overliming and activated charcoal treatments was developed. Two such detoxified process hydrolysates (NREL CF: aspen and CESF: maple) were each fermented with appropriate LA supplementation. For the NREL CF hydrolysate-based cultures amended with 0.25-0.5% LA, P(3HB-co-3HV) yields, PHA contents (PHA as percent of dry biomass), and mol% 3HV compositions of 2.0 g/l, 40% (w/w), and 16-52 mol% were obtained, respectively. Similarly, the CESF hydrolysate-based shake-flask cultures yielded 1.6 g/l PHA, 39% (w/w) PHA contents, and 4-67 mol% 3HV compositions. These data are comparable to copolymer yields and cellular contents reported for hexose plus levulinic acid-based shake-flask cultures, as reported using Alcaligenes eutrophus and Pseudomonas putida. However, our findings presage a conceivable alternative, forestry-based biorefinery approach for the production of value-added biodegradable PHA polymers. Specifically, this review describes the current and potential utilization of lignocellulosic process streams as platform precursors to PHA polymers including hemicellulosic hydrolysates, residual cellulose-derived levulinic acid, tall oil fatty acids (Kraft pulping residual), and lignin-derived aromatics. |
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Authors:
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Thomas M Keenan; James P Nakas; Stuart W Tanenbaum |
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Publication Detail:
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Type: Journal Article; Review Date: 2006-04-27 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: 33 ISSN: 1367-5435 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-08 Completed Date: 2007-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 616-26 Citation Subset: IM |
Affiliation:
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Department of Environmental and Forest Biology, State University of New York, College of Environmental Science and Forestry (SUNY-ESF), Syracuse, NY 13210, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcaligenes
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growth & development,
metabolism Biotechnology* Burkholderia cepacia / growth & development, metabolism* Industrial Microbiology / methods* Polyesters / chemistry, metabolism* Pseudomonas putida / growth & development, metabolism Wood / metabolism, microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Polyesters; 0/poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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