Document Detail

Polyhemoglobin with different percentage of tetrameric hemoglobin and effects on vasoactivity and electrocardiogram.
MedLine Citation:
PMID:  16537172     Owner:  NLM     Status:  MEDLINE    
There has been considerable discussions on why some types of haemoglobin-based blood substitutes increase vasoactivity whereas a very few others do not. In this study, we prepare four different types of PolyHb each containing different percentage of tetrameric hemoglobin using glutaraldehyde crosslinking and characterized to ensure that they all have the same oxygen affinity. Thus the preparations are prepared from the same chemical method and have the same oxygen affinity. We infused these in the form of 1/6 volume toploading into anesthetized rats to simulate the use of blood substitutes in surgery. Mean arterial pressure (MAP) increased immediately after injection of PolyHb containing 38% or 78% of tetrameric hemoglobin. However, there was no significant increase in blood pressure with the injection of PolyHb containing 16% or 0.4% tetrameric hemoglobin. In electrocardiogram (ECG) study, we observe that high percentage (78%) of tetrameric hemoglobin causes marked changes in ECG immediately after infusion. Injection of PolyHb containing 16% or 38% of tetrameric hemoglobin resulted in minimal elevation of the ST segment. Infusion of PolyHb containing 0.4% of tetrameric hemoglobin did not result in any changes.
Binglan Yu; Zunchang Liu; Thomas Ming Swi Chang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial cells, blood substitutes, and immobilization biotechnology     Volume:  34     ISSN:  1073-1199     ISO Abbreviation:  Artif Cells Blood Substit Immobil Biotechnol     Publication Date:  2006  
Date Detail:
Created Date:  2006-03-15     Completed Date:  2006-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431307     Medline TA:  Artif Cells Blood Substit Immobil Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-73     Citation Subset:  IM    
Artificial Cell and Organ Research Center, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
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MeSH Terms
Blood Pressure / drug effects*
Blood Substitutes / administration & dosage*,  chemistry,  pharmacology
Hemoglobins / administration & dosage*,  analysis,  chemistry,  pharmacology
Infusions, Intravenous
Molecular Weight
Oxygen / analysis
Rats, Wistar
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/polyhemoglobin; 7782-44-7/Oxygen

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