| Poly(glucosyl-N-acetylgalactosamine 1-phosphate), a wall teichoic acid of Bacillus subtilis 168: its biosynthetic pathway and mode of attachment to peptidoglycan. | |
| | |
MedLine Citation:
|
PMID: 16735734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The ggaAB operon of Bacillus subtilis 168 encodes enzymes responsible for the synthesis of poly(glucosyl N-acetylgalactosamine 1-phosphate) [poly(GlcGalNAc 1-P)], a wall teichoic acid (WTA). Analysis of the nucleotide sequence revealed that both GgaA and GgaB contained the motif characteristic of sugar transferases, while GgaB was most likely to be bifunctional, being endowed with an additional motif present in glucosyl/glycerophosphate transferases. Transcription of the operon was thermosensitive, and took place from an unusually distant sigma(A)-controlled promoter. The incorporation of the poly(GlcGalNAc 1-P) precursors by various mutants deficient in the synthesis of poly(glycerol phosphate), which is the most abundant WTA of strain 168, revealed that both WTAs were most likely to be attached to peptidoglycan (PG) through the same linkage unit (LU). The incorporation of poly(GlcGalNAc 1-P) precursors by protoplasts confirmed the existence of this LU, and provided further evidence that incorporation takes place at the outer surface of the protoplast membrane. The data presented here strengthen the view that biosynthesis of the LU, and the hooking of the LU-endowed polymer to PG, offer distinct widespread targets for antibiotics specific to Gram-positive bacteria. |
| | |
Authors:
|
Pierre-Philippe Freymond; Vladimir Lazarevic; Blazenka Soldo; Dimitri Karamata |
Related Documents
:
|
9522764 - Etching of young permanent teeth with acid gel--a comparison between the effect of 15 s... 20652374 - Poly(2-hydroxyethyl methacrylate-co-dodecyl methacrylate-co-acrylic acid): synthesis, p... 16851124 - Experimental and modelization approach in the study of acid-site energy distribution by... 15747184 - Porous and dense poly(l-lactic acid) and poly(d,l-lactic acid-co-glycolic acid) scaffol... 16216074 - Crystal structure analysis of phosphatidylcholine-gm2-activator product complexes: evid... 1899804 - Potentiation of arachidonic acid release by phorbol myristate acetate in platelets is n... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: 152 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2006 Jun |
Date Detail:
|
Created Date: 2006-05-31 Completed Date: 2006-08-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
|
Languages: eng Pagination: 1709-18 Citation Subset: IM |
Affiliation:
|
Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
genetics,
metabolism* Bacterial Proteins / genetics Base Sequence Cell Wall / metabolism Glycerophosphates / metabolism Glycosyltransferases / metabolism Molecular Sequence Data Operon Peptidoglycan / metabolism* Teichoic Acids / biosynthesis* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Glycerophosphates; 0/Peptidoglycan; 0/Teichoic Acids; 0/poly(3-O-beta-glucopyranosyl-N-acetylgalactosamine 1-phosphate); EC 2.4.-/Glycosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A generalized transducing phage (phiIF3) for the genomically sequenced Serratia marcescens strain Db...
Next Document: Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SOD...