Document Detail


Polygenic sex determination system in zebrafish.
MedLine Citation:
PMID:  22506019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Despite the popularity of zebrafish as a research model, its sex determination (SD) mechanism is still unknown. Most cytogenetic studies failed to find dimorphic sex chromosomes and no primary sex determining switch has been identified even though the assembly of zebrafish genome sequence is near to completion and a high resolution genetic map is available. Recent publications suggest that environmental factors within the natural range have minimal impact on sex ratios of zebrafish populations. The primary aim of this study is to find out more about how sex is determined in zebrafish.
METHODOLOGY/PRINCIPAL FINDINGS: Using classical breeding experiments, we found that sex ratios across families were wide ranging (4.8% to 97.3% males). On the other hand, repeated single pair crossings produced broods of very similar sex ratios, indicating that parental genotypes have a role in the sex ratio of the offspring. Variation among family sex ratios was reduced after selection for breeding pairs with predominantly male or female offspring, another indication that zebrafish sex is regulated genetically. Further examinations by a PCR-based "blind assay" and array comparative genomic hybridization both failed to find universal sex-linked differences between the male and female genomes. Together with the ability to increase the sex bias of lines by selective breeding, these data suggest that zebrafish is unlikely to utilize a chromosomal sex determination (CSD) system.
CONCLUSIONS/SIGNIFICANCE: Taken together, our study suggests that zebrafish sex is genetically determined with limited, secondary influences from the environment. As we have not found any sign for CSD in the species, we propose that the zebrafish has a polygenic sex determination system.
Authors:
Woei Chang Liew; Richard Bartfai; Zijie Lim; Rajini Sreenivasan; Kellee R Siegfried; Laszlo Orban
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-10
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-16     Completed Date:  2012-10-17     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e34397     Citation Subset:  IM    
Affiliation:
Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Breeding
Comparative Genomic Hybridization / methods
Environment
Female
Genome
Genotype
Male
Multifactorial Inheritance*
Sex Characteristics
Sex Chromosomes
Sex Determination Processes*
Sex Ratio
Zebrafish / genetics*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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