Document Detail


Poly(gamma-L-diaminobutanoic acid), a novel poly(amino acid), coproduced with poly(epsilon-L-lysine) by two strains of Streptomyces celluloflavus.
MedLine Citation:
PMID:  18625024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two poly(epsilon-L-lysine) (epsilon-PL) producer strains of Streptomyces celluloflavus secreted a novel polymeric substance into their culture broths along with epsilon-PL. Three types of HPLC analysis plus one- and two-dimensional 1H and 13C nuclear magnetic resonance experiments revealed that the secreted substance was poly(gamma-L-diaminobutanoic acid) (gamma-PAB), an L-alpha,gamma-diaminobutanoic acid (L-DAB) homopolymer linking between y-amino and alpha-carboxylic acid functional groups. The gamma-PABs from the two strains had an identical chemical structure, and the same number-average molecular weight of 2100-2200. No copolymers composed of the two amino acids L-DAB and L-lysine were found in either of the broths from the producers. Both strains coproduced high levels of the two poly(amino acid)s in the presence of SO4(2-) at pH 4.0 and 4.5 L min(-1) aeration in a 5-L jar fermentor. gamma-PAB exhibited strong inhibitory activities against various yeasts and weaker actions against bacteria than epsilon-PL. gamma-PAB may have various biological functions similar to epsilon-PL, and the use of gamma-PAB along with epsilon-PL would be advantageous for technical applications in various fields.
Authors:
Munenori Takehara; Masayuki Saimura; Haruka Inaba; Hideo Hirohara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  286     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-29     Completed Date:  2008-11-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  110-7     Citation Subset:  IM    
Affiliation:
Department of Materials Science, University of Shiga Prefecture, Hikone, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry*,  metabolism*,  pharmacology
Bacteria / drug effects
Biopolymers / chemistry,  metabolism,  pharmacology
Fermentation
Lysine / chemistry,  metabolism
Streptomyces / chemistry*,  metabolism*
Yeasts / drug effects
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biopolymers; 56-87-1/Lysine

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