| Polyethylenimine strategies for plasmid delivery to brain-derived cells. | |
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MedLine Citation:
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PMID: 15121169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The introduction of effective transfection reagents has had a dramatic impact on basic scientific studies over the past decade and is methodically becoming a clinical relevant agent. An area where these agents have had little impact to date is in transfection of neuronal cells either in vivo or in vitro. The poor results, obtained with these cells, likely arise from the innate properties of the cell itself such as its post-mitotic state and its fragility to the transfection agent. In this report, we investigated the transfection efficiency of branched and linear form of polyethylenimine (PEI) for a commonly used tissue culture cell line, the human CF bronchial epithelial cell line IB3-1, rat brain-derived glial, and neuronal cell lines. In addition, the effect of reaction conditions, such as ratio of PEI/plasmid, polymer molecular weight, and shape, was addressed on the transfection effects. The results indicate that branched PEI is more effective for the brain-derived cells. It is also shown that PEI 25 is more effective for the glial cells and PEI 50-100 is more effective for the neuronal cells under the evaluation conditions. |
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Authors:
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Chun Zhang; Preeti Yadava; Jeffrey Hughes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 33 ISSN: 1046-2023 ISO Abbreviation: Methods Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-05-03 Completed Date: 2004-12-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 144-50 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Elsevier Inc. |
Affiliation:
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Department of Pharmaceutics, University of Florida, Gainesville, FL 32601, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology* Cell Culture Techniques Green Fluorescent Proteins / analysis, genetics Humans Neuroglia / cytology Neurons / cytology Plasmids / chemistry, metabolism* Polyethyleneimine / chemistry* Rats Transfection / methods* |
| Grant Support | |
ID/Acronym/Agency:
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P01 AG 10485/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 9002-98-6/Polyethyleneimine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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