Document Detail


Polyethylenimine strategies for plasmid delivery to brain-derived cells.
MedLine Citation:
PMID:  15121169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The introduction of effective transfection reagents has had a dramatic impact on basic scientific studies over the past decade and is methodically becoming a clinical relevant agent. An area where these agents have had little impact to date is in transfection of neuronal cells either in vivo or in vitro. The poor results, obtained with these cells, likely arise from the innate properties of the cell itself such as its post-mitotic state and its fragility to the transfection agent. In this report, we investigated the transfection efficiency of branched and linear form of polyethylenimine (PEI) for a commonly used tissue culture cell line, the human CF bronchial epithelial cell line IB3-1, rat brain-derived glial, and neuronal cell lines. In addition, the effect of reaction conditions, such as ratio of PEI/plasmid, polymer molecular weight, and shape, was addressed on the transfection effects. The results indicate that branched PEI is more effective for the brain-derived cells. It is also shown that PEI 25 is more effective for the glial cells and PEI 50-100 is more effective for the neuronal cells under the evaluation conditions.
Authors:
Chun Zhang; Preeti Yadava; Jeffrey Hughes
Related Documents :
25439439 - Relationship between automated total nucleated cell count and enumeration of cells on d...
9557679 - The transmembrane domains of sindbis virus envelope glycoproteins induce cell death.
15194799 - Gene transduction and cell entry pathway of fiber-modified adenovirus type 5 vectors ca...
12625759 - Highly efficient, large volume flow electroporation.
3416359 - Expressed recombinant cadherins mediate cell sorting in model systems.
20391019 - A rapid bioassay for recombinant interleukin-22.
22948659 - Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell m...
17030349 - L1-cam in a membrane-bound or soluble form augments protection from apoptosis in ovaria...
23092119 - In vitro demonstration of apoptosis mediated photodynamic activity and nir nucleus imag...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  33     ISSN:  1046-2023     ISO Abbreviation:  Methods     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-03     Completed Date:  2004-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  144-50     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Elsevier Inc.
Affiliation:
Department of Pharmaceutics, University of Florida, Gainesville, FL 32601, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology*
Cell Culture Techniques
Green Fluorescent Proteins / analysis,  genetics
Humans
Neuroglia / cytology
Neurons / cytology
Plasmids / chemistry,  metabolism*
Polyethyleneimine / chemistry*
Rats
Transfection / methods*
Grant Support
ID/Acronym/Agency:
P01 AG 10485/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 9002-98-6/Polyethyleneimine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transfection of adherent and suspended cells by calcium phosphate.
Next Document:  New non-viral method for gene transfer into primary cells.