Document Detail

Polyethylene glycol in conjunction with a second antibody for 24-hour radioimmunoassays of bovine prolactin and growth hormone.
MedLine Citation:
PMID:  6875064     Owner:  NLM     Status:  MEDLINE    
Separate 24-h radioimmunoassays were validated for bovine prolactin and growth hormone using polyethylene glycol in conjunction with a second antibody for separating bound from free labeled hormone. Buffer, standards, sera, antisera, and radioiodinated hormone were added to assay tubes and incubated 24 h. The second antibody was added and incubated for 15 min. Then 1 ml of 6% polyethylene glycol (3% final concentration) was added, and tubes immediately were centrifuged. Polyethylene glycol effectively decreased incubation time. There was no significant difference of percent binding, percent nonspecific binding, or sensitivity of these assays as compared to assays requiring a 72-h incubation with the second antibody when polyethylene glycol was not used. Specificities of the assays were demonstrated by parallelism between varying volumes of bovine serum and standard hormones. Sensitivities of the assays were .4 and .8 ng for prolactin and growth hormone. Intra- and interassay coefficients of variations were 8 and 12% for prolactin and 5 and 18% for growth hormone. Recoveries of known amounts of added hormones were 81 and 75% for prolactin and growth hormone.
J R Eisenman; B P Chew
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  66     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-09-09     Completed Date:  1983-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1174-9     Citation Subset:  IM    
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MeSH Terms
Antigen-Antibody Complex
Binding Sites, Antibody
Growth Hormone / blood*
Hydrogen-Ion Concentration
Polyethylene Glycols / pharmacology
Prolactin / blood*
Radioimmunoassay / methods,  veterinary*
Reg. No./Substance:
0/Antibodies; 0/Antigen-Antibody Complex; 0/Binding Sites, Antibody; 0/Polyethylene Glycols; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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