| Polyelectrolyte multilayer films: A sponge for insulin? | |
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MedLine Citation:
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PMID: 20930331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Considering restrictive diabetes treatments, new insulin administration strategies constitute a huge medical challenge. This study aimed at developing a new support for insulin reservoirs, using polyelectrolyte multilayer films (PEM films), and thus studying this hormone release in a progressive manner. At first, insulin was loaded in (PDADMAC-PAA)n films, by immerging them for various periods of time (2, 14 and 24 h) in a solution containing this protein. Confocal laser scanning microscopy (CLSM) revealed that insulin-FITC could diffuse inside the film with a bigger concentration in the upper part of the film (after 2 and 14 h in contact with the polypeptide solution), and then in the whole film (after 24 h) from a solution at pH=4.3 (below insulin's isolelectric point). Environmental scanning electron microscopy (ESEM) and CLSM showed that the film swells upon insulin loading. We finally investigated the insulin release by ATR-FTIR spectroscopy. It revealed that a loaded (PDADMAC-PAA)15 film, immerged in distilled water, showed no measurable insulin release. In contrast, a slow unloading was observed in the presence of a NaCl 0.15 M solution (salinity close to physiological serum). This study could open the route for a new way of insulin delivery. |
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Authors:
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Nadia Ladhari; Joseph Hemmerlé; Youssef Haikel; Jean-Claude Voegel; Vincent Ball |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bio-medical materials and engineering Volume: 20 ISSN: 1878-3619 ISO Abbreviation: Biomed Mater Eng Publication Date: 2010 |
Date Detail:
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Created Date: 2010-10-08 Completed Date: 2011-02-25 Revised Date: 2011-07-29 |
Medline Journal Info:
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Nlm Unique ID: 9104021 Medline TA: Biomed Mater Eng Country: Netherlands |
Other Details:
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Languages: eng Pagination: 217-25 Citation Subset: IM |
Affiliation:
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Université de Strasbourg, Faculté de Chirurgie Dentaire, Strasbourg, France. Nadia.Ladhari@odonto-ulp.u-strasbg.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Alanine / analogs & derivatives*, chemistry Biocompatible Materials / chemistry* Diffusion Electrolytes / chemistry* Insulin / chemistry* Materials Testing Membranes, Artificial* Polyethylenes / chemistry* Quaternary Ammonium Compounds / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Electrolytes; 0/Membranes, Artificial; 0/Polyethylenes; 0/Quaternary Ammonium Compounds; 11061-68-0/Insulin; 145545-23-9/3-(phenylamino)alanine; 26062-79-3/poly-N,N-dimethyl-N,N-diallylammonium chloride; 56-41-7/Alanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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