Document Detail

Polyelectrolyte coatings for microchip capillary electrophoresis.
MedLine Citation:
PMID:  16790867     Owner:  NLM     Status:  MEDLINE    
In chip-based electrophoretic analysis of biomolecules, chemical modification of the microchannel is widely employed to reduce or eliminate the analyte-wall interactions and alter electroosmotic flow (EOF) in the microchannel. A stable polyelectrolyte multilayer coating is one common way to regulate or eliminate EOF and prevent analyte adsorption for the rapid, efficient separation of biomolecules within microchannels. A wide variety of polyelectrolytes have been used as coatings. This chapter deals with how to coat microchips with polyelectrolytes and the expected results using polybrene and dextran sulfate as models. The technique presented here is generally applicable to any polyelectrolyte.
Yan Liu; Charles S Henry
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  339     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  IM    
Department of Chemistry, Colorado State University, Fort Collins, CO, USA.
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MeSH Terms
Electrophoresis, Microchip / instrumentation*,  methods
Equipment Design
Surface Properties
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Electrolytes; 0/Nylons; 0/Polymers; 0/poly(dimethylsiloxane)-polyamide copolymer

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