| Polyelectrolyte Coating of Iron Oxide Nanoparticles for MRI-based cell tracking. | |
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MedLine Citation:
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PMID: 21893141 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Iron oxide-based magnetic nanoparticles (MNPs) offer unique properties for cell tracking by magnetic resonance imaging (MRI) in cellular immunotherapy. In this study, we investigated the uptake of chemically engineered nanoparticles into antigen-presenting dendritic cells (DCs). DCs are expected to perceive MNPs as foreign antigens, thus exhibiting the capability to immunologically sense MNP surface chemistry. In order to systematically evaluate cellular uptake and T2/T2⁎ MR imaging properties of MNPs, we synthesized polymer-based MNPs by employing layer-by-layer (LbL) technology. Thereby, we achieved modification of particle shell parameters, such as size, surface charge, and chemistry. We found that sub-cellular packaging of MNPs rather than MNP content in DCs influences MR imaging quality. Increased local intracellular electron density as inferred from transmission electron microscopy strongly correlated with enhanced contrast in MRI. Thus, LbL-tailoring of MNP shells using polyelectrolytes that impact on uptake and sub-cellular localization can be used for modulating MR imaging properties. |
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Authors:
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Sebastian Schwarz; John E Wong; Joerg Bornemann; Michael Hodenius; Uwe Himmelreich; Walter Richtering; Mathias Hoehn; Martin Zenke; Thomas Hieronymus |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-2 |
Journal Detail:
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Title: Nanomedicine : nanotechnology, biology, and medicine Volume: - ISSN: 1549-9642 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233142 Medline TA: Nanomedicine Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute for Biomedical Engineering, Department of Cell Biology, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany; Helmholtz Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University, Pauwelsstrasse 20, 52074 Aachen, Germany. |
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