| Polyelectrolyte capsules-containing HIV-1 p24 and poly I:C modulate dendritic cells to stimulate HIV-1-specific immune responses. | |
| | |
MedLine Citation:
|
PMID: 20461059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polyelectrolyte microcapsules (MCs) are potent protein delivery vehicles which can be tailored with ligands to stimulate maturation of dendritic cells (DCs). We investigated the immune stimulatory capacity of monocyte-derived DC (Mo-DC) loaded with these MCs, containing p24 antigen from human immunodeficiency virus type 1 (HIV-1) alone [p24-containing MC (MCp24)] or with the Toll-like receptor ligand 3 (TLR3) ligand poly I:C (MCp24pIC) as a maturation factor. MO-DC, loaded with MCp24pIC, upregulated CCR7, CD80, CD83, and CD86 and produced high amounts of interleukin-12 (IL-12) cytokine, to a similar extent as MCp24 in the presence of an optimized cytokine cocktail. MO-DC from HIV-infected patients under highly active antiretroviral therapy (HAART) exposed to MCp24 together with cytokine cocktail or to MCp24pIC expanded autologous p24-specific CD4(+) and CD8(+) T-cell responses as measured by interferon-gamma (IFN-gamma) and IL-2 cytokine production and secretion. In vivo relevance was shown by immunizing C57BL/6 mice with MCp24pIC, which induced both humoral and cellular p24-specific immune responses. Together these data provide a proof of principle that both antigen and DC maturation signal can be delivered as a complex with polyelectrolyte capsules to stimulate virus-specific T cells both in vitro and in vivo. Polyelectrolyte MCs could be useful for in vivo immunization in HIV-1 and other infections. |
| | |
Authors:
|
Winni De Haes; Stefaan De Koker; Charlotte Pollard; Derek Atkinson; Erika Vlieghe; Jessy Hoste; Joanna Rejman; Stefaan De Smedt; Johan Grooten; Guido Vanham; Ellen Van Gulck |
Related Documents
:
|
12794169 - Failure of monocytes of trauma patients to convert to immature dendritic cells is relat... 16566999 - Differential detergent fractionation for non-electrophoretic bovine peripheral blood mo... 17301819 - Zoledronic acid inhibits the function of toll-like receptor 4 ligand activated monocyte... 20711689 - Human embryonic stem cell-extracts inhibit the differentiation and function of monocyte... 20362279 - Inhibiting effects of total saponins of panax ginseng on immune maturation of dendritic... 19915049 - Absence of the transcriptional repressor blimp-1 in hematopoietic lineages reveals its ... 12529049 - Transgenic models of tolerance and autoimmunity: with special reference to systemic lup... 22099179 - More than nervous: the emerging roles of plexins. 21633179 - Inflammatory links between obesity and metabolic disease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-11 |
Journal Detail:
|
Title: Molecular therapy : the journal of the American Society of Gene Therapy Volume: 18 ISSN: 1525-0024 ISO Abbreviation: Mol. Ther. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-02 Completed Date: 2010-09-30 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 100890581 Medline TA: Mol Ther Country: United States |
Other Details:
|
Languages: eng Pagination: 1408-16 Citation Subset: IM |
Affiliation:
|
Institute of Tropical Medicine of Antwerp, Department of Microbiology, Unit Virology, Antwerp, Belgium. wdehaes@itg.be |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Dendritic Cells / immunology* Drug Carriers / chemistry* Female HIV Core Protein p24 / chemistry, immunology* HIV-1 / immunology Humans Immunity, Cellular / immunology Immunity, Humoral / immunology Interferon-gamma / metabolism Interleukin-12 / metabolism Interleukin-2 / metabolism Mice Mice, Inbred C57BL Microscopy, Fluorescence Poly I-C / chemistry, immunology* Polymers / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Drug Carriers; 0/HIV Core Protein p24; 0/Interleukin-2; 0/Polymers; 187348-17-0/Interleukin-12; 24939-03-5/Poly I-C; 82115-62-6/Interferon-gamma |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S ...
Next Document: The chase for the RIG-I ligand--recent advances.