Document Detail


Polydopamine-enabled surface functionalization of gold nanorods for cancer cell-targeted imaging and photothermal therapy.
MedLine Citation:
PMID:  22891865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: A novel biomimetic strategy was employed for presenting antibodies on gold nanorods (NRs) to target growth factor receptors on cancer cells for use in photothermal therapy.
MATERIALS & METHODS: Polydopamine (PD) was polymerized onto gold NRs, and EGF receptor antibodies (anti-EGFR) were immobilized onto the layer. Cell-binding affinity and light-activated cell death of cancer cells incubated with anti-EGFR-PD-NRs were quantified by optical imaging.
RESULTS: PD was deposited onto gold NRs, and antibodies were bound to PD-coated NRs. Anti-EGFR-PD-NRs were stable in media, and were specifically bound to EGFR-overexpressing cells. Illumination of cells targeted with anti-EGFR-PD-NRs enhanced cell death compared with nonirradiated controls and cells treated with antibody-free NRs.
CONCLUSION: PD facilitates the surface functionalization of gold NRs with biomolecules, allowing cell targeting and photothermal killing of cancer cells. PD can potentially coat a large variety of nanoparticles with targeting ligands as a strategy for biofunctionalization of diagnostic and therapeutic nanoparticles.
Authors:
Kvar C L Black; Ji Yi; José G Rivera; Daria C Zelasko-Leon; Phillip B Messersmith
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Publication Detail:
Type:  Journal Article     Date:  2012-08-14
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  8     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-21     Completed Date:  2013-06-04     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  17-28     Citation Subset:  IM    
Affiliation:
Northwestern University, Evanston, IL 60208, USA.
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MeSH Terms
Descriptor/Qualifier:
Gold / chemistry*
Indoles / chemistry*
Microscopy, Electron, Transmission
Nanotubes*
Neoplasms / pathology*
Polyethylene Glycols / chemistry
Polymers / chemistry*
Spectroscopy, Near-Infrared
Grant Support
ID/Acronym/Agency:
R01 EB005772/EB/NIBIB NIH HHS; R37 DE014193/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Polyethylene Glycols; 0/Polymers; 0/polydopamine; 7440-57-5/Gold
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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