| Polycystic ovary syndrome: evidence for reduced 3 beta-hydroxysteroid dehydrogenase gene expression in human luteinizing granulosa cells. | |
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MedLine Citation:
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PMID: 10813104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase (3 beta-HSD) is an important enzyme for the biosynthesis of progesterone, the reduced capacity of PCO luteinizing granulosa cells to synthesize progesterone in vitro may be due to reduced 3 beta-HSD gene expression. A reverse transcriptase polymerase chain reaction for 3 beta-HSD was performed and the relative intensity of signals for 3 beta-HSD was evaluated using computer-assisted densitometry. Cells from polycystic ovaries expressed less 3 beta-HSD in follicles < or 10 mm (p < 0.05) and in follicles > or 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 beta-HSD in follicles > or = 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in the gene expression of 3 beta-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone. |
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Authors:
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N Doldi; D Grossi; A Destefani; A Gessi; A Ferrari |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology Volume: 14 ISSN: 0951-3590 ISO Abbreviation: Gynecol. Endocrinol. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-07-28 Completed Date: 2000-07-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8807913 Medline TA: Gynecol Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 32-7 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Milan, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxysteroid Dehydrogenases
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genetics* Cells, Cultured Chorionic Gonadotropin / pharmacology Female Gene Expression* Granulosa Cells / drug effects, enzymology* Humans Polycystic Ovary Syndrome / enzymology* Progesterone / secretion RNA / analysis Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 57-83-0/Progesterone; 63231-63-0/RNA; EC 1.1.-/3-Hydroxysteroid Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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