Document Detail

Polycyclic aromatic hydrocarbons suppress meiosis in primordial germ cells via the AHR signaling pathway.
MedLine Citation:
PMID:  22387805     Owner:  NLM     Status:  Publisher    
Numerous studies have demonstrated that polycyclic aromatic hydrocarbon (PAH) exposure during pregnancy is associated with negative reproductive consequences in adulthood, such as reduced sperm production and oocyte destruction in human and laboratory animals. However, the mechanism(s) underlying these epidemiological findings is still unclear, largely due to the lack of an in vitro model of gametogenesis. Here we established an in vitro model of gametogenesis through retinoic acid (RA)-induced differentiation of chicken primordial germ cells (PGCs) that progressed through meiosis to generate 55% of haploid cells. Using this system, we demonstrated that PAH exposure directly suppressed meiotic initiation and completion in chicken PGCs and promoted the apoptosis of PGCs, eventually leading to reduction of haploid cell formation by 66.9%. Furthermore, knockdown experiments suggested that AHR was essential for the adverse effects of PAH on PGC differentiation. Collectively, these findings offer novel insight into the dynamic mechanism by which PAH affects gametogenesis, suggesting that AHR-dependent inhibition of meiosis might be the cause of reduced gamete production. This study also provides a novel model for direct examination of the molecular and genetic pathways of environmental toxicants on gametogenesis.
Chutian Ge; Jian Ye; Qin Wang; Caiqiao Zhang; Jun-Mo Yang; Guoying Qian
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-25
Journal Detail:
Title:  Toxicology letters     Volume:  -     ISSN:  1879-3169     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China; College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
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