| Polycomblike 2 facilitates the recruitment of PRC2 Polycomb group complexes to the inactive X chromosome and to target loci in embryonic stem cells. | |
| | |
MedLine Citation:
|
PMID: 21367819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polycomb group (PcG) proteins play an important role in the control of developmental gene expression in higher organisms. In mammalian systems, PcG proteins participate in the control of pluripotency, cell fate, cell cycle regulation, X chromosome inactivation and parental imprinting. In this study we have analysed the function of the mouse PcG protein polycomblike 2 (Pcl2), one of three homologues of the Drosophila Polycomblike (Pcl) protein. We show that Pcl2 is expressed at high levels during early embryogenesis and in embryonic stem (ES) cells. At the biochemical level, Pcl2 interacts with core components of the histone H3K27 methyltransferase complex Polycomb repressive complex 2 (PRC2), to form a distinct substoichiometric biochemical complex, Pcl2-PRC2. Functional analysis using RNAi knockdown demonstrates that Pcl2-PRC2 facilitates both PRC2 recruitment to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. The role of Pcl2 in PRC2 targeting in ES cells is critically dependent on a conserved PHD finger domain, suggesting that Pcl2 might function through the recognition of a specific chromatin configuration. |
| | |
Authors:
|
Miguel Casanova; Tanja Preissner; Andrea Cerase; Raymond Poot; Daisuke Yamada; Xiangzhi Li; Ruth Appanah; Karel Bezstarosti; Jeroen Demmers; Haruhiko Koseki; Neil Brockdorff |
Related Documents
:
|
2613759 - Synthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis o... 15557119 - Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements... 11479219 - Multiple microtubule alterations are associated with vinca alkaloid resistance in human... 7896879 - Microtubule polarity and dynamics in the control of organelle positioning, segregation,... 20581449 - Centrosomal chk2 in dna damage responses and cell cycle progression. 18155769 - Effect of an experimental proteasome inhibitor on the cytoskeleton, cytosolic protein t... 21227659 - Cell adhesive and growth behavior on electrospun nanofibrous scaffolds by designed mult... 23411669 - Therapeutic effect of 188re-mag3-depreotide on non-small cell lung cancer in vivo and i... 60869 - Calcitonin cells and unusual follicles of the thyroid of the indian grey mongoose, herp... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-02 |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 138 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-03-23 Completed Date: 2011-05-23 Revised Date: 2013-05-26 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 1471-82 Citation Subset: IM |
Affiliation:
|
Developmental Epigenetics Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Cell Line Chromatin Immunoprecipitation Chromatography, Gel Embryonic Stem Cells / metabolism* Female Immunoprecipitation In Situ Hybridization, Fluorescence Male Mass Spectrometry Mice Polycomb Repressive Complex 2 Polycomb-Group Proteins Pregnancy Repressor Proteins / genetics, metabolism* X Chromosome / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
//Medical Research Council; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Mtf2 protein, mouse; 0/Polycomb-Group Proteins; 0/Repressor Proteins; EC 2.1.1.43/Polycomb Repressive Complex 2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clonal analysis by distinct viral vectors identifies bona fide neural stem cells in the adult zebraf...
Next Document: Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Phys...