Document Detail


Polycomblike 2 facilitates the recruitment of PRC2 Polycomb group complexes to the inactive X chromosome and to target loci in embryonic stem cells.
MedLine Citation:
PMID:  21367819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polycomb group (PcG) proteins play an important role in the control of developmental gene expression in higher organisms. In mammalian systems, PcG proteins participate in the control of pluripotency, cell fate, cell cycle regulation, X chromosome inactivation and parental imprinting. In this study we have analysed the function of the mouse PcG protein polycomblike 2 (Pcl2), one of three homologues of the Drosophila Polycomblike (Pcl) protein. We show that Pcl2 is expressed at high levels during early embryogenesis and in embryonic stem (ES) cells. At the biochemical level, Pcl2 interacts with core components of the histone H3K27 methyltransferase complex Polycomb repressive complex 2 (PRC2), to form a distinct substoichiometric biochemical complex, Pcl2-PRC2. Functional analysis using RNAi knockdown demonstrates that Pcl2-PRC2 facilitates both PRC2 recruitment to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. The role of Pcl2 in PRC2 targeting in ES cells is critically dependent on a conserved PHD finger domain, suggesting that Pcl2 might function through the recognition of a specific chromatin configuration.
Authors:
Miguel Casanova; Tanja Preissner; Andrea Cerase; Raymond Poot; Daisuke Yamada; Xiangzhi Li; Ruth Appanah; Karel Bezstarosti; Jeroen Demmers; Haruhiko Koseki; Neil Brockdorff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-02
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  138     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-23     Completed Date:  2011-05-23     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1471-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Line
Chromatin Immunoprecipitation
Chromatography, Gel
Embryonic Stem Cells / metabolism*
Female
Immunoprecipitation
In Situ Hybridization, Fluorescence
Male
Mass Spectrometry
Mice
Polycomb Repressive Complex 2
Polycomb-Group Proteins
Pregnancy
Repressor Proteins / genetics,  metabolism*
X Chromosome / metabolism*
Grant Support
ID/Acronym/Agency:
081385//Wellcome Trust; //Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Mtf2 protein, mouse; 0/Polycomb-Group Proteins; 0/Repressor Proteins; EC 2.1.1.43/Polycomb Repressive Complex 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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