Document Detail

Polycomb repressive complex 2 impedes intestinal cell terminal differentiation.
MedLine Citation:
PMID:  22467857     Owner:  NLM     Status:  MEDLINE    
The crypt-villus axis constitutes the functional unit of the small intestine, where mature absorptive cells are confined to the villi, and stem cells and transit amplifying and differentiating cells are restricted to the crypts. The polycomb group (PcG) proteins repress differentiation and promote self-renewal in embryonic stem cells. PcGs prevent transcriptional activity by catalysing epigenetic modifications, such as the covalent addition of methyl groups on histone tails, through the action of the polycomb repressive complex 2 (PRC2). Although a role for PcGs in the preservation of stemness characteristics is now well established, recent evidence suggests that they may also be involved in the regulation of differentiation. Using intestinal epithelial cell models that recapitulate the enterocytic differentiation programme, we generated a RNAi-mediated stable knockdown of SUZ12, which constitutes a cornerstone for PRC2 assembly and functionality, in order to analyse intestinal cell proliferation and differentiation. Expression of SUZ12 was also investigated in human intestinal tissues, revealing the presence of SUZ12 in most proliferative epithelial cells of the crypt and an increase in its expression in colorectal cancers. Moreover, PRC2 disruption led to a significant precocious expression of a number of terminal differentiation markers in intestinal cell models. Taken together, our data identified a mechanism whereby PcG proteins participate in the repression of the enterocytic differentiation program, and suggest that a similar mechanism exists in situ to slow down terminal differentiation in the transit amplifying cell population.
Yannick D Benoit; Manon B Lepage; Taoufik Khalfaoui; Eric Tremblay; Nuria Basora; Julie C Carrier; Lorraine J Gudas; Jean-François Beaulieu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-30
Journal Detail:
Title:  Journal of cell science     Volume:  125     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-09-07     Completed Date:  2013-03-25     Revised Date:  2013-07-16    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  3454-63     Citation Subset:  IM    
CIHR Team on the Digestive Epithelium, Département d'Anatomie et Biologie Cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, QC, Canada.
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MeSH Terms
Caco-2 Cells
Cell Differentiation / physiology
Cell Growth Processes / physiology
Cells, Cultured
Gastrointestinal Tract / cytology*,  metabolism
Gene Expression Regulation, Developmental
Histones / genetics,  metabolism
Polycomb Repressive Complex 2 / genetics,  metabolism,  physiology*
Promoter Regions, Genetic
Transcription, Genetic
Grant Support
MOP-123415//Canadian Institutes of Health Research; R01 CA0043796-22/CA/NCI NIH HHS; R01 CA043796/CA/NCI NIH HHS; R01 DE010389/DE/NIDCR NIH HHS
Reg. No./Substance:
0/Histones; EC Repressive Complex 2

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