Document Detail


Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and is required for S phase progression.
MedLine Citation:
PMID:  18453536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polycomb group (PcG) proteins form two distinct complexes, PRC1 and PRC2, to regulate developmental target genes by maintaining the epigenetic state in cells. PRC2 methylates histone H3 at lysine 27 (H3K27), and PRC1 then recognizes methyl-H3K27 to form repressive chromatin. However, it remains unknown how PcG proteins maintain stable and plastic chromatin during cell division. Here we report that PcG-associated chromatin is reproduced in the G(1) phase in post-mitotic cells and is required for subsequent S phase progression. In dividing cells, H3K27 trimethylation (H3K27Me(3)) marked mitotic chromosome arms where PRC2 (Suz12 and Ezh2) co-existed, whereas PRC1 (Bmi1 and Pc2) appeared in distinct foci in the pericentromeric regions. As each PRC complex was increasingly assembled from mitosis to G(1) phase, PRC1 formed H3K27Me(3)-based chromatin intensively during middle and late G(1) phase; this chromatin was highly resistant to in situ nuclease treatment. Thus, the transition from mitosis to G(1) phase is crucial for PcG-mediated chromatin inheritance. Knockdown of Suz12 markedly reduced the amount of H3K27Me(3) on mitotic chromosomes, and as a consequence, PRC1 foci were not fully transmitted to post-mitotic daughter cells. S phase progression was markedly delayed in these Suz12-knockdown cells. The fact that PcG-associated chromatin is reproduced during post-mitotic G(1) phase suggests the possibility that PcG proteins enable their target chromatin to be remodeled in response to stimuli in the G(1) phase.
Authors:
Takahiro Aoto; Noriko Saitoh; Yasuo Sakamoto; Sugiko Watanabe; Mitsuyoshi Nakao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-02
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  283     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-30     Completed Date:  2008-08-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18905-15     Citation Subset:  IM    
Affiliation:
Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatin / genetics,  metabolism*
Chromatin Assembly and Disassembly / physiology*
DNA-Binding Proteins / genetics,  metabolism
Epigenesis, Genetic / physiology
G1 Phase / physiology*
Hela Cells
Histone-Lysine N-Methyltransferase
Histones / genetics,  metabolism
Humans
Methylation
Mice
Mitosis / physiology
Multiprotein Complexes / genetics,  metabolism*
NIH 3T3 Cells
Proteins / genetics,  metabolism
Repressor Proteins / genetics,  metabolism*
S Phase / physiology*
Transcription Factors / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA-Binding Proteins; 0/EZH2 protein, human; 0/Histones; 0/Multiprotein Complexes; 0/Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/polycomb group proteins; EC 2.1.1.-/Ezh2 protein, mouse; EC 2.1.1.43/Histone-Lysine N-Methyltransferase

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