Document Detail


Polyclonal in vitro T cell proliferation and T cell-dependent B cell differentiation supported by activated autologous B cells.
MedLine Citation:
PMID:  7517348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although anti-CD3-induced T cell proliferation and T cell-dependent B cell differentiation are not supported well by resting (nonactivated) B cells as AC, human peripheral blood B cells activated in vitro with SAC, rIL2, or anti-IgM effectively subserve AC function in a manner qualitatively similar to that of blood monocytes and support polyclonal anti-CD3-driven T cell-dependent B cell differentiation. Physical contact among T cells, responder B cells, and (irradiated) activated B cells is required, and the ability of activated B cells to support anti-CD3-driven generation of IgSC parallels surface B7 expression by the activated B cells. The fusion protein CTLA4Ig, which binds to B7 and B7-like molecules with high avidity and disrupts the interaction of T cell surface CD28 with B7, inhibits B cell differentiation in a dose-dependent fashion, whereas a control fusion protein has no such effect. Thus, activated B cells support polyclonal T cell-dependent B cell differentiation via a CD28 (CTLA4)/B7 (B7-like)-dependent mechanism.
Authors:
W Stohl; J E Elliott; P S Linsley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical immunology and immunopathology     Volume:  72     ISSN:  0090-1229     ISO Abbreviation:  Clin. Immunol. Immunopathol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-29     Completed Date:  1994-07-29     Revised Date:  2013-05-24    
Medline Journal Info:
Nlm Unique ID:  0356637     Medline TA:  Clin Immunol Immunopathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  44-52     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Southern California School of Medicine, Los Angeles 90033.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD
Antigens, CD28 / immunology
Antigens, CD3 / immunology
Antigens, CD80 / immunology
Antigens, Differentiation / immunology
B-Lymphocytes / physiology*
CTLA-4 Antigen
Cell Communication / immunology
Cell Differentiation / immunology
Cells, Cultured
Humans
Immunoconjugates*
Interleukin-2 / immunology
Lymphocyte Activation / immunology*
T-Lymphocytes / physiology*
Grant Support
ID/Acronym/Agency:
AR41006/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD28; 0/Antigens, CD3; 0/Antigens, CD80; 0/Antigens, Differentiation; 0/CTLA-4 Antigen; 0/CTLA4 protein, human; 0/Immunoconjugates; 0/Interleukin-2; 7D0YB67S97/abatacept

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