| Polyclonal antibodies to a recombinant coat protein of Potato virus A. | |
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MedLine Citation:
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PMID: 12580376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector pMPM4omega. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a titer of 1:1024 in ELISA and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA). |
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Authors:
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N Cerovská; T Moravec; J Velemínský |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta virologica Volume: 46 ISSN: 0001-723X ISO Abbreviation: Acta Virol. Publication Date: 2002 |
Date Detail:
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Created Date: 2003-02-12 Completed Date: 2003-03-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370401 Medline TA: Acta Virol Country: Slovakia |
Other Details:
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Languages: eng Pagination: 147-51 Citation Subset: IM |
Affiliation:
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Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, 160 00 Prague 6, Czech Republic. cerovska@ueb.cas.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Antibodies, Viral / analysis, immunology* Blotting, Western Capsid Proteins / genetics, immunology* Cloning, Molecular Enzyme-Linked Immunosorbent Assay Escherichia coli / metabolism Microscopy, Electron Potyvirus / genetics, immunology* Recombinant Proteins / chemistry, immunology Solanum tuberosum / virology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Capsid Proteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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