Document Detail


Polycations increase the permeability of Mycobacterium vaccae cell envelopes to hydrophobic compounds.
MedLine Citation:
PMID:  11577156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polycations [protamine, polymyxin B nonapeptide (PMBN) and polyethyleneimine (PEI)] have been shown to increase the cell wall permeability of Mycobacterium vaccae to highly hydrophobic compounds, as manifested in enhanced intracellular bioconversion of beta-sitosterol to 4-androsten-3,17-dione (AD) and 1,4-androstadien-3,17-dione (ADD), and cell sensitization to erythromycin and rifampicin. The quantity of AD(D) formed per biomass unit was twice as high in the presence of PMBN and PEI, and three times higher with protamine. The sensitization factor, i.e. the MIC(50) ratio of the control bacteria to those exposed to polycations, ranged from 4 to 16, depending on the polycation/antibiotic combination. Non-covalently bound free lipids were extracted from the control and polycation-treated cells and fractionated with the use of chloroform, acetone and methanol. Chloroform- and acetone-eluted fractions (mainly neutral lipids and glycolipids, respectively) showed significant polycation-induced alterations in their quantitative and qualitative composition. The fatty acid profile of neutral lipids was reduced in comparison to control, whereas acetone-derived lipids were characterized by a much higher level of octadecenoic acid (C(18:1)) and a considerably lower content of docosanoic acid (C(22:0)), the marker compound of mycolate-containing glycolipids. Methanol-eluted fractions remained unaltered. Cell-wall-linked mycolates obtained from delipidated cells were apparently unaffected by the action of polycations, as judged from the TLC pattern of mycolic acid subclasses, the mean weight of mycolate preparations and the C(22:0) acid content in the mycolates, determined by GC/MS and pyrolysis GC. The results suggest the involvement of the components of non-covalently bound lipids in the outer layer in the M. vaccae permeability barrier.
Authors:
M Korycka-Machala; A Ziółkowski; A Rumijowska-Galewicz; K Lisowska; L Sedlaczek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  147     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-28     Completed Date:  2002-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2769-81     Citation Subset:  IM    
Affiliation:
Centre for Microbiology & Virology, Polish Academy of Sciences, 93-232 Lód, Lodowa 106, Poland.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology
Cell Membrane Permeability / drug effects*
Cell Wall / chemistry,  drug effects,  metabolism*
Erythromycin / pharmacology
Fatty Acids / analysis
Microbial Sensitivity Tests
Mycobacterium / chemistry,  drug effects,  metabolism*
Mycolic Acids / analysis
Polyamines / pharmacology*
Rifampin / pharmacology
Sitosterols / metabolism
Surface Properties
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fatty Acids; 0/Mycolic Acids; 0/Polyamines; 0/Sitosterols; 0/polycations; 114-07-8/Erythromycin; 13292-46-1/Rifampin; 5779-62-4/sitosterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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