| Polyamines and nucleic acid metabolism in chick embryo. Incorporation of labelled precursors into nucleic acids of subcellular fractions and polyribosomal patterns. | |
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MedLine Citation:
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PMID: 16742581 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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1. An increase in polyamine concentration, caused by inhibiting the amine oxidase activities with iproniazid, increased the incorporation of [(3)H]orotic acid into chick-embryo RNA and DNA. On the other hand, a decrease in polyamine concentration, obtained by causing an increase in amine oxidase activities, decreased [(3)H]orotic acid incorporation into nucleic acids. This was particularly evident for nuclear DNA and ribosomal RNA. 2. Polyribosomal patterns obtained by sucrose-density-gradient centrifugation showed highest radioactivity in the regions of 259s and 280s aggregates in those embryos in which the polyamine contents were enhanced, whereas a decrease in the radioactivity was observed when the polyamine concentrations were decreased. 3. The activity of DNA-dependent RNA polymerase, assayed in the same experimental conditions, also varied in the same fashion with changes in polyamine concentration. |
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Authors:
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G Moruzzi; B Barbiroli; C M Caldarera |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 107 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1968 May |
Date Detail:
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Created Date: 2010-06-25 Completed Date: 2010-06-25 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 609-13 Citation Subset: - |
Affiliation:
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Istituto di Chimica Biologica dell'Università di Bologna, Bologna, Italy. |
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