Document Detail


Polyamine oxidase, a hydrogen peroxide-producing enzyme, is up-regulated by light and down-regulated by auxin in the outer tissues of the maize mesocotyl.
MedLine Citation:
PMID:  12586904     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exogenously supplied auxin (1-naphthaleneacetic acid) inhibited light-induced activity increase of polyamine oxidase (PAO), a hydrogen peroxide-producing enzyme, in the outer tissues of maize (Zea mays) mesocotyl. The same phenomenon operates at PAO protein and mRNA accumulation levels. The wall-bound to extractable PAO activity ratio was unaffected by auxin treatment, either in the dark or after light exposure. Ethylene treatment did not affect PAO activity, thus excluding an effect of auxin via increased ethylene biosynthesis. The auxin polar transport inhibitors N(1)-naphthylphthalamic acid or 2,3,5-triiodobenzoic acid caused a further increase of PAO expression in outer tissues after light treatment. The small increase of PAO expression, normally occurring in the mesocotyl epidermis during plant development in the dark, was also inhibited by auxin, although to a lesser extent with respect to light-exposed tissue, and was stimulated by N(1)-naphthylphthalamic acid or 2,3,5-triiodobenzoic acid, thus suggesting a complex regulation of PAO expression. Immunogold ultrastructural analysis in epidermal cells revealed the association of PAO with the secretory pathway and the cell walls. The presence of the enzyme in the cell walls of this tissue greatly increased in response to light treatment. Consistent with auxin effects on light-induced PAO expression, the hormone treatment inhibited the increase in immunogold staining both intraprotoplasmically and in the cell wall. These results suggest that both light and auxin finely tune PAO expression during the light-induced differentiation of the cell wall in the maize mesocotyl epidermal tissues.
Authors:
Alessandra Cona; Francesco Cenci; Manuela Cervelli; Rodolfo Federico; Paolo Mariottini; Sandra Moreno; Riccardo Angelini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  131     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-05-23     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  803-13     Citation Subset:  IM    
Affiliation:
Dipartimento di Biologia, Università degli Studi "Roma Tre," Viale Guglielmo Marconi 446, 00146 Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Enzyme Activation / genetics,  radiation effects
Ethylenes / pharmacology
Gene Expression Regulation, Enzymologic / drug effects,  radiation effects
Gene Expression Regulation, Plant / drug effects,  radiation effects
Hydrogen Peroxide / metabolism*
Immunohistochemistry
Indoleacetic Acids / pharmacology*
Light
Microscopy, Immunoelectron
Oxidoreductases Acting on CH-NH Group Donors / genetics*,  metabolism
Phthalimides / pharmacology
Plant Epidermis / cytology,  enzymology,  ultrastructure
RNA, Messenger / genetics,  metabolism
Seeds / enzymology*,  genetics
Triiodobenzoic Acids / pharmacology
Zea mays / enzymology*,  genetics
Chemical
Reg. No./Substance:
0/Ethylenes; 0/Indoleacetic Acids; 0/Phthalimides; 0/RNA, Messenger; 0/Triiodobenzoic Acids; 132-66-1/alpha-naphthylphthalamic acid; 74-85-1/ethylene; 7722-84-1/Hydrogen Peroxide; 88-82-4/2,3,5-triiodobenzoic acid; EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors; EC 1.5.3.-/polyamine oxidase
Comments/Corrections

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