| Polyamine deficiency leads to accumulation of reactive oxygen species in a spe2Delta mutant of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 16862607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that polyamine-deficient Saccharomyces cerevisiae are very sensitive to incubation in oxygen. The current studies show that, even under more physiological conditions (i.e. growth in air), polyamine-deficient cells accumulate reactive oxygen species (ROS). These cells develop an apoptotic phenotype and, after incubation in polyamine-deficient medium, die. To show a specific effect of polyamines on ROS accumulation, uncomplicated by any effects on growth, spermine was added to spermidine-deficient spe2Delta fms1Delta cells, since spermine does not affect the growth of this strain. In this strain, spermine addition caused a marked, but not complete, decrease in the accumulation of ROS and a moderate protection against cell death. In other experiments with polyamine-deficient cells containing plasmids that overexpress superoxide dismutases (SOD1, SOD2), ROS decreased but with only a partial protection against cell death. Polyamine-deficient cells incubated anaerobically show markedly less cell death. These data show that part of the function of polyamines is protection of the cells from accumulation of ROS. |
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Authors:
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Manas K Chattopadhyay; Celia White Tabor; Herbert Tabor |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Yeast (Chichester, England) Volume: 23 ISSN: 0749-503X ISO Abbreviation: Yeast Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-31 Completed Date: 2006-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8607637 Medline TA: Yeast Country: England |
Other Details:
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Languages: eng Pagination: 751-61 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2006 John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory of Biochemistry and Genetics, Building 8, Room 223, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology Fluoresceins / pharmacology Fluorescent Dyes / pharmacology Microscopy, Confocal Microscopy, Fluorescence Mutation Reactive Oxygen Species / metabolism* Rhodamines / pharmacology Saccharomyces cerevisiae / enzymology, genetics, metabolism* Spermidine / metabolism* Spermine / pharmacology* Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Fluorescent Dyes; 0/Reactive Oxygen Species; 0/Rhodamines; 109244-58-8/dihydrorhodamine 123; 124-20-9/Spermidine; 71-44-3/Spermine; 76-54-0/2',7'-dichlorofluorescein; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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