Document Detail


Polyamidoamine dendrimers surface-engineered with biomimetic phosphorylcholine as potential drug delivery carriers.
MedLine Citation:
PMID:  21237622     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biomimetic acryloyloxyethyl phosphorylcholine (APC) was used to react with generation 5 poly(amido amine) (PAMAM) dendrimers (G5) via the Michael addition reaction between primary amino group of PAMAM dendrimers and acrylic functional group of APC. FTIR and (1)H NMR confirmed the success of surface modification of G5. The primary amino and phosphorylcholine (PC) group numbers of the surface engineered PAMAM dendrimers (G5-PC) were calculated to be 56 and 50 via (1)H NMR and potentiometric titration. Cell viability and cell morphology studies indicated that biomimetic phosphorylcholine surface engineering successfully lowered the cytotoxicity of G5 PAMAM dendrimers. The hydrophobic interior of G5-PC was used to incorporate anti-cancer drug Adriamycin (ADR) and the G5-PC showed sustained releasing behavior for ADR. Cell morphology and viability tests indicated that the drug-loaded G5-PC conjugate could effectively enter the cancer cells and inhibit the growth of cancer cells. Biomimetic phosphorylcholine surface engineered PAMAM dendrimers with lowered cytotoxicity and high cellular penetrating ability showed great potential for the biomedical applications as nanocarrier system.
Authors:
Lan Jia; Jian-Ping Xu; Hai Wang; Jian Ji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-14
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  84     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-02-21     Completed Date:  2011-06-01     Revised Date:  2011-09-27    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  49-54     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, Zhejiang Province, China.
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MeSH Terms
Descriptor/Qualifier:
Biomimetics
Cell Line, Tumor
Cell Survival / drug effects
Dendrimers / chemistry*,  pharmacology
Drug Delivery Systems*
Humans
Models, Biological
Phosphorylcholine / chemistry*,  pharmacology
Surface Properties
Chemical
Reg. No./Substance:
0/Dendrimers; 0/PAMAM Starburst; 107-73-3/Phosphorylcholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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